Drought and recovery in barley: key gene networks and retrotransposon response

Frontiers in Plant Science

Plant Science

Wei Chang¹  Marko Jääskeläinen¹  Maitry Paul¹  Alan H. Schulman²  Jaakko Tanskanen²  Menachem Moshelion³  Ahan Dalal³ 

[1] HiLIFE Institute of Biotechnology, University of Helsinki, Helsinki, Finland;Viikki Plant Science Centre (ViPS), University of Helsinki, Helsinki, Finland;HiLIFE Institute of Biotechnology, University of Helsinki, Helsinki, Finland;Viikki Plant Science Centre (ViPS), University of Helsinki, Helsinki, Finland;Production Systems, Natural Resources Institute Finland (LUKE), Helsinki, Finland;The Robert H. Smith Institute of Plant Sciences and Genetics in Agriculture, The Hebrew University of Jerusalem, Rehovot, Israel;

关键词: drought;    autophagy;    barley;    resilience (environmental);    network analysis;    gene expression;    Hordeum vulgare;   

DOI  :  10.3389/fpls.2023.1193284

 received in 2023-03-24, accepted in 2023-05-09,  发布年份 2023

来源: Frontiers

【 摘 要 】

IntroductionDuring drought, plants close their stomata at a critical soil water content (SWC), together with making diverse physiological, developmental, and biochemical responses.MethodsUsing precision-phenotyping lysimeters, we imposed pre-flowering drought on four barley varieties (Arvo, Golden Promise, Hankkija 673, and Morex) and followed their physiological responses. For Golden Promise, we carried out RNA-seq on leaf transcripts before and during drought and during recovery, also examining retrotransposon BARE1expression. Transcriptional data were subjected to network analysis.ResultsThe varieties differed by their critical SWC (ϴcrit), Hankkija 673 responding at the highest and Golden Promise at the lowest. Pathways connected to drought and salinity response were strongly upregulated during drought; pathways connected to growth and development were strongly downregulated. During recovery, growth and development pathways were upregulated; altogether, 117 networked genes involved in ubiquitin-mediated autophagy were downregulated.DiscussionThe differential response to SWC suggests adaptation to distinct rainfall patterns. We identified several strongly differentially expressed genes not earlier associated with drought response in barley. BARE1 transcription is strongly transcriptionally upregulated by drought and downregulated during recovery unequally between the investigated cultivars. The downregulation of networked autophagy genes suggests a role for autophagy in drought response; its importance to resilience should be further investigated.

【 授权许可】

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Copyright © 2023 Paul, Tanskanen, Jääskeläinen, Chang, Dalal, Moshelion and Schulman