Transcriptome data analysis of primary cardiomyopathies reveals perturbations in arachidonic acid metabolism

Frontiers in Cardiovascular Medicine

Cardiovascular Medicine

Pankaj Kumar Chauhan¹  Ramanathan Sowdhamini² 

[1] National Centre for Biological Sciences (Tata Institute of Fundamental Research), Bangalore, India;National Centre for Biological Sciences (Tata Institute of Fundamental Research), Bangalore, India;Molecular Biophysics Unit, Indian Institute of Science, Bangalore, India;Institute of Bioinformatics and Applied Biotechnology, Bangalore, India;

关键词: cardiomyopathies;    GSA;    metabolism;    transcriptome;    heart failure;    arachidonic acid;   

DOI  :  10.3389/fcvm.2023.1110119

 received in 2022-11-28, accepted in 2023-05-09,  发布年份 2023

来源: Frontiers

【 摘 要 】

IntroductionCardiomyopathies are complex heart diseases with significant prevalence around the world. Among these, primary forms are the major contributors to heart failure and sudden cardiac death. As a high-energy demanding engine, the heart utilizes fatty acids, glucose, amino acid, lactate and ketone bodies for energy to meet its requirement. However, continuous myocardial stress and cardiomyopathies drive towards metabolic impairment that advances heart failure (HF) pathogenesis. So far, metabolic profile correlation across different cardiomyopathies remains poorly understood.MethodsIn this study, we systematically explore metabolic differences amongst primary cardiomyopathies. By assessing the metabolic gene expression of all primary cardiomyopathies, we highlight the significantly shared and distinct metabolic pathways that may represent specialized adaptations to unique cellular demands. We utilized publicly available RNA-seq datasets to profile global changes in the above diseases (|log2FC| ≥ 0.28 and BH adjusted p-val 0.1) and performed gene set analysis (GSA) using the PAGE statistics on KEGG pathways.ResultsOur analysis demonstrates that genes in arachidonic acid metabolism (AA) are significantly perturbed across cardiomyopathies. In particular, the arachidonic acid metabolism gene PLA2G2A interacts with fibroblast marker genes and can potentially influence fibrosis during cardiomyopathy.ConclusionThe profound significance of AA metabolism within the cardiovascular system renders it a key player in modulating the phenotypes of cardiomyopathies.

【 授权许可】

Unknown   
© 2023 Chauhan and Sowdhamini.