Transcriptome and metabolome analyses reveal that Bacillus subtilis BS-Z15 lipopeptides mycosubtilin homologue mediates plant defense responses

Frontiers in Plant Science

Plant Science

Shengcheng Han¹  Heping Zhao¹  Qi Zhang¹  Qilin Yang²  Huixin Zhao²  Reyihanguli Aimaier²  Jia You²  Hui Zhang²  Jun Yang²  Jingbo Zhang²  Jinjin Zhao² 

[1] Beijing Key Laboratory of Gene Resource and Molecular Development, College of Life Sciences, Beijing Normal University, Beijing, China;Xinjiang Key Laboratory of Special Species Conservation and Regulatory Biology, College of Life Science, Xinjiang Normal University, Urumqi, China;

关键词: plant-microbial interactions;    systemic resistance;    B. subtilis BS-Z15;    mycosubtilin homologue;    RNA-Seq;    GC-MS;   

DOI  :  10.3389/fpls.2022.1088220

 received in 2022-11-03, accepted in 2022-12-30,  发布年份 2023

来源: Frontiers

【 摘 要 】

Microbial-plant interactions protect plants from external stimuli, releasing various elicitor that activate the plants defense response and regulate its growth. Bacillus subtilis BS-Z15 was screened from cotton inter-rhizosphere soil, antagonized various plant pathogens, and protected cotton against Verticillium dahliae. This study showed that the BS-Z15 lipopeptide mycosubtilin homologue could act as an elicitor to induce systemic resistance (ISR) in plants. Mycosubtilin homologue induced ROS burst and deposition, callose deposition, MAPK cascade phosphorylation, and up-regulated PR1 and PDF1.2 gene expression in Arabidopsis seedlings, moreover enhanced resistance of Arabidopsis to Pseudomonas syringae pv. Tomato DC3000 (Pst DC3000) and V. dahliae. Transcriptome analysis was then used to evaluate the impact of mycosubtilin homologue on plant gene expression control. Mycosubtilin homologues activated Arabidopsis ISR on genes in metabolic pathways such as Arabidopsis plant-pathogen interactions, phenylpropanoid biosynthesis, MAPK signaling pathway, and phytohormone signaling. These analyses revealed that mycosubtilin homologues mediate the regulation of plant systemic resistance and growth and development by affecting related metabolites in glycolysis and gluconeogenesis, pentose phosphate pathway, tricarboxylic acid cycle, and amino acid metabolism in Arabidopsis. These findings confirmed that a mycosubtilin homologue could trigger the initiation of the Arabidopsis ISR by interacting with a variety of PTI components and transcriptional metabolic signaling pathways.

【 授权许可】

Unknown   
Copyright © 2023 Yang, Zhang, You, Yang, Zhang, Zhao, Aimaier, Zhang, Han, Zhao and Zhao