Maternal vaginal microbiome composition does not affect development of the infant gut microbiome in early life

Frontiers in Cellular and Infection Microbiology

Cellular and Infection Microbiology

Tim J. Dumonceaux¹  Soren Gantt²  Matthew G. Links³  Zahra Pakzad⁴  Deborah M. Money⁵  Julie E. Van Schalkwyk⁶  Jennifer A. Hutcheon⁶  K.S. Joseph⁶  Janet Lyons⁶  Kirsten Grabowska⁶  Sheona M. Mitchell-Foster⁶  Janet E. Hill⁷  Scott J. Dos Santos⁷  Zoë G. Hodgson⁸  Amee R. Manges⁹  Arianne Y. K. Albert^1,10  Evelyn J. Maan^1,10  Chelsea N. Elwood^1,11 

[1] Agriculture and Agri-Food Canada, Saskatoon, SK, Canada;Centre de Recherche du CHU Sainte-Justine, Montréal, QC, Canada;Department of Animal and Poultry Science, University of Saskatchewan, Saskatoon, SK, Canada;Department of Computer Science, University of Saskatchewan, Saskatoon, SK, Canada;Department of Microbiology and Immunology, Faculty of Science, University of British Columbia, Vancouver, BC, Canada;Women’s Health Research Institute, B.C. Women's Hopsital, Vancouver, BC, Canada;Department of Microbiology and Immunology, Faculty of Science, University of British Columbia, Vancouver, BC, Canada;Women’s Health Research Institute, B.C. Women's Hopsital, Vancouver, BC, Canada;Department of Obstetrics and Gynaecology, Faculty of Medicine, University of British Columbia, Vancouver, BC, Canada;Department of Obstetrics and Gynaecology, Faculty of Medicine, University of British Columbia, Vancouver, BC, Canada;Department of Veterinary Microbiology, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, SK, Canada;Midwifery Program, Faculty of Medicine, University of British Columbia, Vancouver, BC, Canada;School of Population and Public Health, University of British Columbia, Vancouver, BC, Canada;British Columbia Centre for Disease Control, Vancouver, BC, Canada;Women’s Health Research Institute, B.C. Women's Hopsital, Vancouver, BC, Canada;Women’s Health Research Institute, B.C. Women's Hopsital, Vancouver, BC, Canada;Department of Obstetrics and Gynaecology, Faculty of Medicine, University of British Columbia, Vancouver, BC, Canada;

关键词: vaginal microbiome;    infant stool microbiome;    infant gut;    cpn;    vaginal seeding;    birth mode;    microbiome;   

DOI  :  10.3389/fcimb.2023.1144254

 received in 2023-01-14, accepted in 2023-03-07,  发布年份 2023

来源: Frontiers

【 摘 要 】

Birth mode has been implicated as a major factor influencing neonatal gut microbiome development, and it has been assumed that lack of exposure to the maternal vaginal microbiome is responsible for gut dysbiosis among caesarean-delivered infants. Consequently, practices to correct dysbiotic gut microbiomes, such as vaginal seeding, have arisen while the effect of the maternal vaginal microbiome on that of the infant gut remains unknown. We conducted a longitudinal, prospective cohort study of 621 Canadian pregnant women and their newborn infants and collected pre-delivery maternal vaginal swabs and infant stool samples at 10-days and 3-months of life. Using cpn60-based amplicon sequencing, we defined vaginal and stool microbiome profiles and evaluated the effect of maternal vaginal microbiome composition and various clinical variables on the development of the infant stool microbiome. Infant stool microbiomes showed significant differences in composition by delivery mode at 10-days postpartum; however, this effect could not be explained by maternal vaginal microbiome composition and was vastly reduced by 3 months. Vaginal microbiome clusters were distributed across infant stool clusters in proportion to their frequency in the overall maternal population, indicating independence of the two communities. Intrapartum antibiotic administration was identified as a confounder of infant stool microbiome differences and was associated with lower abundances of Escherichia coli, Bacteroides vulgatus, Bifidobacterium longum and Parabacteroides distasonis. Our findings demonstrate that maternal vaginal microbiome composition at delivery does not affect infant stool microbiome composition and development, suggesting that practices to amend infant stool microbiome composition focus factors other than maternal vaginal microbes.

【 授权许可】

Unknown   
Copyright © 2023 Dos Santos, Pakzad, Albert, Elwood, Grabowska, Links, Hutcheon, Maan, Manges, Dumonceaux, Hodgson, Lyons, Mitchell-Foster, Gantt, Joseph, Van Schalkwyk, Hill and Money