Comprehensive transcriptomic analysis of age-, dark-, and salt-induced senescence reveals underlying mechanisms and key regulators of leaf senescence in Zoysia japonica

Frontiers in Plant Science

Plant Science

Phan Phuong Thao Doan¹  Nguyen Nguyen Chuong¹  Lanshuo Wang¹  Jeongsik Kim²  Jin Hee Kim³  Hyo-Yeon Lee⁴ 

[1] Interdisciplinary Graduate Program in Advanced Convergence Technology & Science, Jeju National University, Jeju, Republic of Korea;Interdisciplinary Graduate Program in Advanced Convergence Technology & Science, Jeju National University, Jeju, Republic of Korea;Subtropical Horticulture Research Institute, Jeju National University, Jeju, Republic of Korea;Faculty of Science Education, Jeju National University, Jeju, Republic of Korea;Subtropical Horticulture Research Institute, Jeju National University, Jeju, Republic of Korea;Subtropical Horticulture Research Institute, Jeju National University, Jeju, Republic of Korea;Department of Biotechnology, Jeju National University, Jeju, Republic of Korea;

关键词: leaf senescence;    transcription factor;    transcriptome analysis;    RNA-Seq;    age;    dark;    salt;    Zoysiagrass;   

DOI  :  10.3389/fpls.2023.1170808

 received in 2023-02-21, accepted in 2023-03-27,  发布年份 2023

来源: Frontiers

【 摘 要 】

The lawn grass Zoysia japonica is widely cultivated for its ornamental and recreational value. However, its green period is subject to shortening, which significantly decreases the economic value of Z. japonica, especially for large cultivations. Leaf senescence is a crucial biological and developmental process that significantly influences the lifespan of plants. Moreover, manipulation of this process can improve the economic value of Z. japonica by extending its greening period. In this study, we conducted a comparative transcriptomic analysis using high-throughput RNA sequencing (RNA-seq) to investigate early senescence responses triggered by age, dark, and salt. Gene set enrichment analysis results indicated that while distinct biological processes were involved in each type of senescence response, common processes were also enriched across all senescence responses. The identification and validation of differentially expressed genes (DEGs) via RNA-seq and quantitative real-time PCR provided up- and down-regulated senescence markers for each senescence and putative senescence regulators that trigger common senescence pathways. Our findings revealed that the NAC, WRKY, bHLH, and ARF transcription factor (TF) groups are major senescence-associated TF families that may be required for the transcriptional regulation of DEGs during leaf senescence. In addition, we experimentally validated the senescence regulatory function of seven TFs including ZjNAP, ZjWRKY75, ZjARF2, ZjNAC1, ZjNAC083, ZjARF1, and ZjPIL5 using a protoplast-based senescence assay. This study provides new insight into the molecular mechanisms underlying Z. japonica leaf senescence and identifies potential genetic resources for enhancing its economic value by prolonging its green period.

【 授权许可】

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Copyright © 2023 Wang, Doan, Chuong, Lee, Kim and Kim