Comparative analysis of phyto-fabricated chitosan, copper oxide, and chitosan-based CuO nanoparticles: antibacterial potential against Acinetobacter baumannii isolates and anticancer activity against HepG2 cell lines

Frontiers in Microbiology

Microbiology

Abeer Hashem¹  Turki M. Dawoud¹  Muhammad Zubair²  Muhammad Hussnain Siddique²  Muhammad Farrukh Sarfraz³  Asma Ashraf⁴  Graciela Dolores Avila-Quezada⁵  Saima Muzammil⁶  Mohsin Khurshid⁶  Muhammad Hassan Sarfraz⁶  Sumreen Hayat⁶  Bilal Aslam⁶  Jorrdy Neves Cruz⁷  Elsayed Fathi Abd_Allah⁸ 

[1] Botany and Microbiology Department, College of Science, King Saud University, Riyadh, Saudi Arabia;Department of Bioinformatics and Biotechnology, Government College University, Faisalabad, Pakistan;Department of Physics, COMSATS University Islamabad, Lahore, Pakistan;Department of Zoology, Government College University, Faisalabad, Pakistan;Facultad de Ciencias Agrotecnológicas, Universidad Autónoma de Chihuahua, Chihuahua, Mexico;Institute of Microbiology, Government College University, Faisalabad, Pakistan;Laboratory of Functional and Structural Biology, Institute of Biological Sciences, Federal University of Pará, Belém, Brazil;Plant Production Department, College of Food and Agricultural Sciences, King Saud University, Riyadh, Saudi Arabia;

关键词: Acinetobacter baumannii;    chitosan;    copper oxide;    nanoparticle;    antimicrobial activity;    anticancer activity;   

DOI  :  10.3389/fmicb.2023.1188743

 received in 2023-03-21, accepted in 2023-05-04,  发布年份 2023

来源: Frontiers

【 摘 要 】

The aim of this study was to provide a comparative analysis of chitosan (CH), copper oxide (CuO), and chitosan-based copper oxide (CH-CuO) nanoparticles for their application in the healthcare sector. The nanoparticles were synthesized by a green approach using the extract of Trianthema portulacastrum. The synthesized nanoparticles were characterized using different techniques, such as the synthesis of the particles, which was confirmed by UV–visible spectrometry that showed absorbance at 300 nm, 255 nm, and 275 nm for the CH, CuO, and CH-CuO nanoparticles, respectively. The spherical morphology of the nanoparticles and the presence of active functional groups was validated by SEM, TEM, and FTIR analysis. The crystalline nature of the particles was verified by XRD spectrum, and the average crystallite sizes of 33.54 nm, 20.13 nm, and 24.14 nm were obtained, respectively. The characterized nanoparticles were evaluated for their in vitro antibacterial and antibiofilm potential against Acinetobacter baumannii isolates, where potent activities were exhibited by the nanoparticles. The bioassay for antioxidant activity also confirmed DPPH scavenging activity for all the nanoparticles. This study also evaluated anticancer activities of the CH, CuO, and CH-CuO nanoparticles against HepG2 cell lines, where maximum inhibitions of 54, 75, and 84% were recorded, respectively. The anticancer activity was also confirmed by phase contrast microscopy, where the treated cells exhibited deformed morphologies. This study demonstrates the potential of the CH-CuO nanoparticle as an effective antibacterial agent, having with its antibiofilm activity, and in cancer treatment.

【 授权许可】

Unknown   
Copyright © 2023 Sarfraz, Zubair, Aslam, Ashraf, Siddique, Hayat, Cruz, Muzammil, Khurshid, Sarfraz, Hashem, Dawoud, Avila-Quezada and Abd_Allah.