RNA-seq transcriptome profiling of pigs’ liver in response to diet with different sources of fatty acids

Frontiers in Genetics

Genetics

Dorian Garrick¹  James Eugene Koltes²  Dawn Koltes²  James Mark Reecy²  Bárbara Silva-Vignato³  Severino Matias de Alencar³  Julia Dezen Gomes³  Luiz Lehmann Coutinho³  Felipe André Oliveira Freitas³  Gerson Barreto Mourão³  Albino Luchiari Filho³  Bruna Pereira Martins da Silva³  Júlio Cesar de Carvalho Balieiro⁴  Vivian Vezzoni de Almeida⁵  Luciana Correia de Almeida Regitano⁶  Juliana Afonso⁶  Mariah Castro Durval⁷  Heidge Fukumasu⁷  Simara Larissa Fanalli⁷  Aline Silva Mello Cesar⁸  Gabriel Costa Monteiro Moreira⁹ 

[1] AL Rae Centre for Genetics and Breeding, Massey University, Hamilton, New Zealand;Animal Science Department, Iowa State University, Ames, IA, United States;Animal Science Department, Luiz de Queiroz College of Agriculture (ESALQ), University of São Paulo, Piracicaba, Brazil;College of Veterinary Medicine and Animal Science, University of São Paulo, Pirassununga, Brazil;Department of Animal Science, College of Veterinary Medicine and Animal Science, Goiânia, Brazil;Embrapa Pecuária Sudeste, São Carlos, Brazil;Faculty of Animal Science and Food Engineering, (FZEA), University of São Paulo, São Paulo, Brazil;Faculty of Animal Science and Food Engineering, (FZEA), University of São Paulo, São Paulo, Brazil;Animal Science Department, Luiz de Queiroz College of Agriculture (ESALQ), University of São Paulo, Piracicaba, Brazil;Unit of Animal Genomics, GIGA Medical Genomics, University of Liège, Liège, Brazil;

关键词: RNA-Seq;    metabolic diseases;    transcriptome;    metabolic disease;    neurodegenerative diseases;    liver;    pig;   

DOI  :  10.3389/fgene.2023.1053021

 received in 2022-09-24, accepted in 2023-01-09,  发布年份 2023

来源: Frontiers

【 摘 要 】

Pigs (Sus scrofa) are an animal model for metabolic diseases in humans. Pork is an important source of fatty acids (FAs) in the human diet, as it is one of the most consumed meats worldwide. The effects of dietary inclusion of oils such as canola, fish, and soybean oils on pig gene expression are mostly unknown. Our objective was to evaluate FA composition, identify changes in gene expression in the liver of male pigs fed diets enriched with different FA profiles, and identify impacted metabolic pathways and gene networks to enlighten the biological mechanisms’ variation. Large White male pigs were randomly allocated to one of three diets with 18 pigs in each; all diets comprised a base of corn and soybean meal to which either 3% of soybean oil (SOY), 3% canola oil (CO), or 3% fish oil (FO) was added for a 98-day trial during the growing and finishing phases. RNA sequencing was performed on the liver samples of each animal by Illumina technology for differential gene expression analyses, using the R package DESeq2. The diets modified the FA profile, mainly in relation to polyunsaturated and saturated FAs. Comparing SOY vs. FO, 143 differentially expressed genes (DEGs) were identified as being associated with metabolism, metabolic and neurodegenerative disease pathways, inflammatory processes, and immune response networks. Comparing CO vs. SOY, 148 DEGs were identified, with pathways related to FA oxidation, regulation of lipid metabolism, and metabolic and neurodegenerative diseases. Our results help explain the behavior of genes with differential expression in metabolic pathways resulting from feeding different types of oils in pig diets.

【 授权许可】

Unknown   
Copyright © 2023 Fanalli, Silva, Gomes, Durval, Almeida, Moreira, Silva-Vignato, Afonso, Freitas, Reecy, Koltes, Koltes, Garrick, Correia de Almeida Regitano, Balieiro, Mourão, Coutinho, Fukumasu, Alencar, Luchiari Filho and Cesar.