Dahuang—Taoren, a botanical drug combination, ameliorates adenomyosis via inhibiting Rho GTPases

Frontiers in Pharmacology

Pharmacology

Li Peng¹  Ya Lei²  Ping Mao³  Xianyun Fu³  Minmin Chen³  Yongli Yi³  Zhao Qu³ 

[1] The First College of Clinical Medical Science, China Three Gorges University, Yichang Central People’s Hospital, Yichang, China;The First College of Clinical Medical Science, China Three Gorges University, Yichang Central People’s Hospital, Yichang, China;Third-Grade Pharmacological Laboratory on Chinese Medicine Approved by State Administration of Traditional Chinese Medicine, Medical College, China Three Gorges University, Yichang, China;Third-Grade Pharmacological Laboratory on Chinese Medicine Approved by State Administration of Traditional Chinese Medicine, Medical College, China Three Gorges University, Yichang, China;

关键词: adenomyosis;    dahuang -taoren combination (DT);    RhoA;    Cdc42;    ROCK1;    Rac1;   

DOI  :  10.3389/fphar.2023.1089004

 received in 2022-11-03, accepted in 2023-02-20,  发布年份 2023

来源: Frontiers

【 摘 要 】

Introduction: Dahuang-Taoren (DT) is a classic combination of botanical drugs applied to treat pain-related diseases in ancient China. Today, DT is frequently applied for dysmenorrhea of adenomyosis (AM) in the clinic. Growing evidence indicates Rho GTPases may play an essential role in AM progression. However, the potential mechanism of DT on Rho GTPases in AM remains unclear.Methods: The expressions of Rho GTPases in the patients with AM were evaluated. Further, pituitary transplantation-induced AM mice and the primary AM endometrial stromal cells (AMESCs) were subjected to DT intervention.Results: The results revealed that the expressions of Rho GTPases were significantly upregulated in both AM patients and AM mice. The DT could reduce pathological infiltration, relieve hyperalgesia, and alleviate cytoskeleton remodeling in AM mice. Besides, the migration and invasion of AMESCs were markedly inhibited after exposure to DT.Discussion: These effects may be linked to the decreased Rho GTPases expression. The results may offer a novel explanation of DT against AM.

【 授权许可】

Unknown   
Copyright © 2023 Lei, Fu, Chen, Yi, Mao, Peng and Qu.