Technological advances for analyzing the content of organ-on-a-chip by mass spectrometry

Frontiers in Bioengineering and Biotechnology

Bioengineering and Biotechnology

Tiffany Porta Siegel¹  Maarten Honing¹  Eva Cuypers¹  Ilona Tosheva¹  Darya Hadavi² 

[1] Maastricht Multimodal Molecular Imaging (M4i) Institute, Division of Imaging Mass Spectrometry Maastricht University, Maastricht, Netherlands;null;

关键词: organ-on-a-chip;    mass spectromelry;    real-time analyis;    online analysis;    analytical technique;    interface;   

DOI  :  10.3389/fbioe.2023.1197760

 received in 2023-03-31, accepted in 2023-05-05,  发布年份 2023

来源: Frontiers

【 摘 要 】

Three-dimensional (3D) cell cultures, including organ-on-a-chip (OOC) devices, offer the possibility to mimic human physiology conditions better than 2D models. The organ-on-a-chip devices have a wide range of applications, including mechanical studies, functional validation, and toxicology investigations. Despite many advances in this field, the major challenge with the use of organ-on-a-chips relies on the lack of online analysis methods preventing the real-time observation of cultured cells. Mass spectrometry is a promising analytical technique for real-time analysis of cell excretes from organ-on-a-chip models. This is due to its high sensitivity, selectivity, and ability to tentatively identify a large variety of unknown compounds, ranging from metabolites, lipids, and peptides to proteins. However, the hyphenation of organ-on-a-chip with MS is largely hampered by the nature of the media used, and the presence of nonvolatile buffers. This in turn stalls the straightforward and online connection of organ-on-a-chip outlet to MS. To overcome this challenge, multiple advances have been made to pre-treat samples right after organ-on-a-chip and just before MS. In this review, we summarised these technological advances and exhaustively evaluated their benefits and shortcomings for successful hyphenation of organ-on-a-chip with MS.

【 授权许可】

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Copyright © 2023 Hadavi, Tosheva, Siegel, Cuypers and Honing.