Chromosome-level reference genome assembly provides insights into the evolution of Pennisetum alopecuroides

Frontiers in Plant Science

Plant Science

Yuesen Yue¹  Ke Teng¹  Qiang Guo¹  Xincun Hou¹  Hui Zhang¹  Yidi Guo¹  Lingyun Liu¹  Wenjun Teng¹  Chunqiao Zhao¹  Xifeng Fan¹  Haifeng Wen¹  Juying Wu¹  Yue Xu¹ 

[1] Institute of Grassland, Flowers, and Ecology, Beijing Academy of Agriculture and Forestry Sciences, Beijing, China;

关键词: P. alopecuroides;    de novo;    comparative genomics;    whole-genome duplication;    forage grass;   

DOI  :  10.3389/fpls.2023.1195479

 received in 2023-03-28, accepted in 2023-07-28,  发布年份 2023

来源: Frontiers

【 摘 要 】

Pennisetum alopecuroides is an important forage grass resource, which plays a vital role in ecological environment improvement. Therefore, the acquisition of P. alopecuroides genome resources is conducive to the study of the adaptability of Pennisetum species in ecological remediation and forage breeding development. Here we assembled a P. alopecuroides cv. 'Liqiu' genome at the chromosome level with a size of approximately 845.71 Mb, contig N50 of 84.83Mb, and genome integrity of 99.13% as assessed by CEGMA. A total of 833.41-Mb sequences were mounted on nine chromosomes by Hi-C technology. In total, 60.66% of the repetitive sequences and 34,312 genes were predicted. The genomic evolution analysis showed that P. alopecuroides cv. 'Liqiu' was isolated from Setaria 7.53–13.80 million years ago and from Cenchrus 5.33–8.99 million years ago, respectively. The whole-genome event analysis showed that P. alopecuroides cv. 'Liqiu' underwent two whole-genome duplication (WGD) events in the evolution process, and the duplication events occurred at a similar time to that of Oryza sativa and Setaria viridis. The completion of the genome sequencing of P. alopecuroides cv. 'Liqiu' provides data support for mining high-quality genetic resources of P. alopecuroides and provides a theoretical basis for the origin and evolutionary characteristics of Pennisetum.

【 授权许可】

Unknown   
Copyright © 2023 Teng, Guo, Liu, Guo, Xu, Hou, Teng, Zhang, Zhao, Yue, Wen, Wu and Fan