In vitro modeling of hepatocellular carcinoma niche on decellularized tomato thorny leaves: a novel natural three-dimensional (3D) scaffold for liver cancer therapeutics

Frontiers in Bioengineering and Biotechnology

Bioengineering and Biotechnology

Naser Farrokhi¹  Mehdi Jahanfar¹  Mariye Ahmadian²  Hosein Shahsavarani²  Atefeh Alipour³  Saadi Hosseini⁴  Shahin Homaeigohar⁵ 

[1] Department of Cell and Molecular Biology, Faculty of Life Science and Biotechnology, Shahid Beheshti University, Tehran, Iran;Department of Cell and Molecular Biology, Faculty of Life Science and Biotechnology, Shahid Beheshti University, Tehran, Iran;Laboratory of Regenerative Medicine and Biomedical Innovations, Pasteur Institute of Iran, National Cell Bank, Tehran, Iran;Department of Nanobiotechnology, Pasteur Institute of Iran, Tehran, Iran;Laboratory of Regenerative Medicine and Biomedical Innovations, Pasteur Institute of Iran, National Cell Bank, Tehran, Iran;School of Science and Engineering, University of Dundee, Dundee, United Kingdom;

关键词: liver cancer modeling;    tumor microenvironment;    3D culture;    natural cellulose scaffolds;    drug screening;   

DOI  :  10.3389/fbioe.2023.1189726

 received in 2023-03-19, accepted in 2023-04-24,  发布年份 2023

来源: Frontiers

【 摘 要 】

Liver cancer is now one of the main causes leading to death worldwide. To achieve reliable therapeutic effects, it is crucial to develop efficient approaches to test novel anticancer drugs. Considering the significant contribution of tumor microenvironment to cell’s response to medications, in vitro 3D bioinspiration of cancer cell niches can be regarded as an advanced strategy to improve the accuracy and reliability of the drug-based treatment. In this regard, decellularized plant tissues can perform as suitable 3D scaffolds for mammalian cell culture to create a near-to-real condition to test drug efficacy. Here, we developed a novel 3D natural scaffold made from decellularized tomato hairy leaves (hereafter called as DTL) to mimic the microenvironment of human hepatocellular carcinoma (HCC) for pharmaceutical purposes. The surface hydrophilicity, mechanical properties, and topography measurement and molecular analyses revealed that the 3D DTL scaffold is an ideal candidate for liver cancer modeling. The cells exhibited a higher growth and proliferation rate within the DTL scaffold, as verified by quantifying the expression of related genes, DAPI staining, and SEM imaging of the cells. Moreover, prilocaine, an anticancer drug, showed a higher effectiveness against the cancer cells cultured on the 3D DTL scaffold, compared to a 2D platform. Taken together, this new cellulosic 3D scaffold can be confidently proposed for chemotherapeutic testing of drugs on hepatocellular carcinoma.

【 授权许可】

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Copyright © 2023 Ahmadian, Hosseini, Alipour, Jahanfar, Farrokhi, Homaeigohar and Shahsavarani.