Comparative transcriptomics reveals the role of altered energy metabolism in the establishment of single-cell C4 photosynthesis in Bienertia sinuspersici

Frontiers in Plant Science

Plant Science

Sang-Yun Han¹  Inhwan Hwang¹  Woe-Yeon Kim²  Jung Sun Kim³ 

[1] Department of Life Sciences, Pohang University of Science and Technology, Pohang, Republic of Korea;Division of Applied Life Science (BK21+) and Research Institute of Life Science, Institute of Agriculture and Life Sciences, Gyeongsang National University, Jinju, Republic of Korea;Genomic Division, Department of Agricultural Bio-Resources, National Institute of Agricultural Sciences, Rural Development Administration, Jeonju, Republic of Korea;

关键词: Bienertia sinuspersici;    Suaeda aralocaspica;    Amaranthus hypochondriacus;    single-cell C4 photosynthesis;    transcriptome;    dimorphic chloroplast;    mitochondria;    malate valve;   

DOI  :  10.3389/fpls.2023.1202521

 received in 2023-04-08, accepted in 2023-05-31,  发布年份 2023

来源: Frontiers

【 摘 要 】

Single-cell C4 photosynthesis (SCC4) in terrestrial plants without Kranz anatomy involves three steps: initial CO2 fixation in the cytosol, CO2 release in mitochondria, and a second CO2 fixation in central chloroplasts. Here, we investigated how the large number of mechanisms underlying these processes, which occur in three different compartments, are orchestrated in a coordinated manner to establish the C4 pathway in Bienertia sinuspersici, a SCC4 plant. Leaves were subjected to transcriptome analysis at three different developmental stages. Functional enrichment analysis revealed that SCC4 cycle genes are coexpressed with genes regulating cyclic electron flow and amino/organic acid metabolism, two key processes required for the production of energy molecules in C3 plants. Comparative gene expression profiling of B. sinuspersici and three other species (Suaeda aralocaspica, Amaranthus hypochondriacus, and Arabidopsis thaliana) showed that the direction of metabolic flux was determined via an alteration in energy supply in peripheral chloroplasts and mitochondria via regulation of gene expression in the direction of the C4 cycle. Based on these results, we propose that the redox homeostasis of energy molecules via energy metabolism regulation is key to the establishment of the SCC4 pathway in B. sinuspersici.

【 授权许可】

Unknown   
Copyright © 2023 Han, Kim, Kim and Hwang