Protective efficacy of Toxoplasma gondii GRA12 or GRA7 recombinant proteins encapsulated in PLGA nanoparticles against acute Toxoplasma gondii infection in mice

Frontiers in Cellular and Infection Microbiology

Cellular and Infection Microbiology

Rong-hui Xie¹  Jing Huang¹  Hong-chao Sun²  Yuan Fu²  Tuan-yuan Shi²  Meng Qi³  Jin-hua Deng³  Pu-ming Deng³ 

[1] Department of Animal Epidemic Surveillance, Zhejiang Provincial Animal Disease Prevention and Control Center, Hangzhou, China;Institute of Animal Husbandry and Veterinary Medicine, Department of Animal Parasitology, Zhejiang Academy of Agricultural Sciences, Hangzhou, China;Institute of Animal Science and Technology, Department of Animal Diseases Diagnosis and Control of Xinjiang Production & Construction Corps, Tarim University, Alar, China;

关键词: GRA7;    GRA12;    nanoparticles;    PLGA;    Toxoplasma gondii;    vaccine;   

DOI  :  10.3389/fcimb.2023.1209755

 received in 2023-04-21, accepted in 2023-06-26,  发布年份 2023

来源: Frontiers

【 摘 要 】

BackgroundToxoplasma gondii is an apicomplexan parasite that affects the health of humans and livestock, and an effective vaccine is urgently required. Nanoparticles can modulate and improve cellular and humoral immune responses.MethodsIn the current study, poly (D, L-lactic-co-glycolic acid) (PLGA) nanoparticles were used as a delivery system for the T. gondii dense granule antigens GRA12 and GRA7. BALB/c mice were injected with the vaccines and protective efficacy was evaluated.ResultsMice immunized with PLGA+GRA12 exhibited significantly higher IgG, and a noticeable predominance of IgG2a over IgG1 was also observed. There was a 1.5-fold higher level of lymphocyte proliferation in PLGA+GRA12-injected mice compared to Alum+GRA12-immunized mice. Higher levels of IFN-g and IL-10 and a lower level of IL-4 were detected, indicating that Th1 and Th2 immune responses were induced but the predominant response was Th1. There were no significant differences between Alum+GRA7-immunized and PLGA+GRA7-immunized groups. Immunization with these four vaccines resulted in significantly reduced parasite loads, but they were lowest in PLGA+GRA12-immunized mice. The survival times of mice immunized with PLGA+GRA12 were also significantly longer than those of mice in the other vaccinated groups.ConclusionThe current study indicated that T. gondii GRA12 recombinant protein encapsulated in PLGA nanoparticles is a promising vaccine against acute toxoplasmosis, but PLGA is almost useless for enhancing the immune response induced by T. gondii GRA7 recombinant protein.

【 授权许可】

Unknown   
Copyright © 2023 Sun, Deng, Fu, Deng, Xie, Huang, Qi and Shi