Multivesicular release favors short term synaptic depression in hippocampal autapses

Frontiers in Cellular Neuroscience

Cellular Neuroscience

Pablo Martínez San Segundo¹  Beatrice Terni¹  Artur Llobet¹ 

[1] Laboratory of Neurobiology, Department of Pathology and Experimental Therapy, Institute of Neurosciences, University of Barcelona, Barcelona, Spain;Bellvitge Biomedical Research Institute (IDIBELL), Barcelona, Spain;

关键词: presynaptic terminal;    synaptic vesicle;    neurotransmitter release;    short-term synaptic depression;    autapse;   

DOI  :  10.3389/fncel.2023.1057242

 received in 2022-09-29, accepted in 2023-04-10,  发布年份 2023

来源: Frontiers

【 摘 要 】

Presynaptic terminals of the central nervous system can support univesicular and multivesicular synchronous release of neurotransmitters, however, the functional implications of the prevalence of one mechanism over the other are yet unresolved. Here, we took advantage of the expression of SF-iGluSnFR.S72A in the astrocytic feeder layer of autaptic hippocampal neuronal cultures to associate the liberation of glutamate to excitatory postsynaptic currents. The presence of the glutamate sensor in glial cells avoided any interference with the function of endogenous postsynaptic receptors. It was possible to optically detect changes in neurotransmitter release probability, which was heterogeneous among synaptic boutons studied. For each neuron investigated, the liberation of neurotransmitters occurred through a predominant mechanism. The prevalence of multivesicular over univesicular release increased synaptic strength and enhanced short-term synaptic depression. These results show that the preference of hippocampal boutons to synchronously release one or more vesicles determines the strength and low pass filtering properties of the synapses established.

【 授权许可】

Unknown   
Copyright © 2023 Martínez San Segundo, Terni and Llobet.