A minimally manipulated preservation and virus inactivation method for amnion/chorion

Frontiers in Bioengineering and Biotechnology

Bioengineering and Biotechnology

Yuyan Ma¹  Xiaoliang Wang²  Jie Wen²  Shang Zhang³  Yu Cheng³  Yongli Yan³  Pin Wang³  Chunxia Zhang³  Lichang Gao³  Changfeng Liu³  Chengru Zhao³  Changlin Liu³ 

[1] Department of Gynecology and Obstetrics, Qilu Hospital, Cheeloo College of Medicine, Shandong University, Jinan, China;Liangchen Biotechnology (Suzhou) Co., Ltd., Suzhou, China;Success Bio-Tech Co., Ltd., Biomedical Material Engineering Laboratory of Shandong Province, Jinan, China;

关键词: diabetic foot ulcer (DFU);    chronic wound;    animal model;    amnion;    virus inactivation;    growth factor (GF);   

DOI  :  10.3389/fbioe.2022.952498

 received in 2022-05-25, accepted in 2022-07-18,  发布年份 2022

来源: Frontiers

【 摘 要 】

Allogeneic amnion tissues have been widely used in tissue repair and regeneration, especially a remarkable trend of clinical uses in chronic wound repair. The virus inactivation procedures are necessary and required to be verified for the clinical use and approval of biological products. Cobalt-60 (Co-60) or electron-beam (e-beam) is the common procedure for virus and bacterial reduction, but the excessive dose of irradiation was reported to be harmful to biological products. Herein, we present a riboflavin (RB)-ultraviolet light (UV) method for virus inactivation of amnion and chorion tissues. We used the standard in vitro limiting dilution assay to test the viral reduction capacity of the RB-UV method on amnion or chorion tissues loaded with four types of model viruses. We found RB-UV was a very effective procedure for inactivating viruses of amnion and chorion tissues, which could be used as a complementary method to Co-60 irradiation. In addition, we also screened the washing solutions and drying methods for the retention of growth factors.

【 授权许可】

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Copyright © 2022 Zhang, Gao, Wang, Ma, Wang, Wen, Cheng, Liu, Zhang, Liu, Yan and Zhao.