Simultaneous editing of two DMR6 genes in grapevine results in reduced susceptibility to downy mildew

Frontiers in Plant Science

Plant Science

Tieme Zeilmaker¹  Jeroen Rouppe van der Voort¹  Oscar Giovannini²  Pietro Franceschi²  Claudio Moser²  Umberto Salvagnin²  Lisa Giacomelli²  Simone Scintilla²  Urska Vrhovsek²  Domenico Masuero² 

[1] Enza Zaden Research & Development B.V., Enkhuizen, Netherlands;Research and Innovation Centre, Fondazione Edmund Mach, San Michele all’Adige, Italy;

关键词: gene editing;    DMR6;    Vitis vinifera;    downy mildew;    susceptibility gene;    Plasmopara viticola;    salicylic acid;   

DOI  :  10.3389/fpls.2023.1242240

 received in 2023-06-18, accepted in 2023-07-17,  发布年份 2023

来源: Frontiers

【 摘 要 】

The reduction of pesticide treatments is of paramount importance for the sustainability of viticulture, and it can be achieved through a combination of strategies, including the cultivation of vines (Vitis vinifera) that are resistant or tolerant to diseases such as downy mildew (DM). In many crops, the knock-out of Downy Mildew Resistant 6 (DMR6) proved successful in controlling DM-resistance, but the effect of mutations in DMR6 genes is not yet known in grapevine.Today, gene editing serves crop improvement with small and specific mutations while maintaining the genetic background of commercially important clones. Moreover, recent technological advances allowed to produce non-transgenic grapevine clones by regeneration of protoplasts edited with the CRISPR/Cas9 ribonucleoprotein. This approach may revolutionize the production of new grapevine varieties and clones, but it requires knowledge about the targets and the impact of editing on plant phenotype and fitness in different cultivars.In this work we generated single and double knock-out mutants by editing DMR6 susceptibility (S) genes using CRISPR/Cas9, and showed that only the combined mutations in VviDMR6-1 and VviDMR6-2 are effective in reducing susceptibility to DM in two table-grape cultivars by increasing the levels of endogenous salicylic acid. Therefore, editing both genes may be necessary for effective DM control in real-world agricultural settings, which could potentially lead to unwanted phenotypes. Additional research, including trials conducted in experimental vineyards, is required to gain a deeper understanding of DMR6-based resistance.

【 授权许可】

Unknown   
Copyright © 2023 Giacomelli, Zeilmaker, Giovannini, Salvagnin, Masuero, Franceschi, Vrhovsek, Scintilla, Rouppe van der Voort and Moser