Plant-produced RBD and cocktail-based vaccine candidates are highly effective against SARS-CoV-2, independently of its emerging variants

Frontiers in Plant Science

Plant Science

Gulshan Mammadova¹  Gulnara Hasanova¹  Merve Ilgin¹  Damla Yuksel¹  Burcu Gulec¹  Irem Gurbuzaslan¹  Tarlan Mamedov²  Muhammet Ali Uygut³  Shaikh Terkis Islam Pavel⁴  Busra Kaplan⁴  Aykut Ozdarendeli⁴  Hazel Yetiskin⁴ 

[1] Department of Agricultural Biotechnology, Akdeniz University, Antalya, Türkiye;Department of Agricultural Biotechnology, Akdeniz University, Antalya, Türkiye;Institute of Molecular Biology and Biotechnologies, Ministry of Science and Education, Republic of Azerbaijan, Baku, Azerbaijan;Department of Microbiology, Medical Faculty, Erciyes University, Kayseri, Türkiye;Department of Microbiology, Medical Faculty, Erciyes University, Kayseri, Türkiye;Vaccine Research, Development and Application Center, Erciyes University, Kayseri, Türkiye;

关键词: SARS-CoV-2;    plant produced RBD;    antigen cocktail;    virus neutralization;    Delta;    Omicron;   

DOI  :  10.3389/fpls.2023.1202570

 received in 2023-04-08, accepted in 2023-07-12,  发布年份 2023

来源: Frontiers

【 摘 要 】

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a novel and highly pathogenic coronavirus that caused an outbreak in Wuhan City, China, in 2019 and then spread rapidly throughout the world. Although several coronavirus disease 2019 (COVID-19) vaccines are currently available for mass immunization, they are less effective against emerging SARS-CoV-2 variants, especially the Omicron (B.1.1.529). Recently, we successfully produced receptor-binding domain (RBD) variants of the spike (S) protein of SARS-CoV-2 and an antigen cocktail in Nicotiana benthamiana, which are highly produced in plants and elicited high-titer antibodies with potent neutralizing activity against SARS-CoV-2. In this study, based on neutralization ability, we demonstrate that plant-produced RBD and cocktail-based vaccine candidates are highly effective against SARS-CoV-2, independently of its emerging variants. These data demonstrate that plant-produced RBD and cocktail-based proteins are the most promising vaccine candidates and may protect against Delta and Omicron-mediated COVID-19. This is the first report describing vaccines against SARS-CoV-2, which demonstrate significant activities against Delta and Omicron variants.

【 授权许可】

Unknown   
Copyright © 2023 Mamedov, Yuksel, Gurbuzaslan, Ilgin, Gulec, Mammadova, Ozdarendeli, Pavel, Yetiskin, Kaplan, Uygut and Hasanova