Sleep deprivation, sleep fragmentation, and social jet lag increase temperature preference in Drosophila

Frontiers in Neuroscience

Neuroscience

Paul J. Shaw¹  Irene Huang¹  Melanie C. Ford¹  Dina Abdalla¹  Hamza Farah¹  Vikram Simhambhatla¹  Zhaoyi Li¹  Erica M. Periandri¹  Vasilios Loutrianakis¹  Arjan Kalra¹  S. Tanner Roach² 

[1] Department of Neuroscience, Washington University School of Medicine, St. Louis, MO, United States;Department of Physiology and Biophysics, University of Illinois at Chicago, Chicago, IL, United States;Department of Neuroscience, Washington University School of Medicine, St. Louis, MO, United States;

关键词: sleep;    sleep deprivation;    Drosophila;    temperature preference behavior;    sleep fragmentation;   

DOI  :  10.3389/fnins.2023.1175478

 received in 2023-02-27, accepted in 2023-05-02,  发布年份 2023

来源: Frontiers

【 摘 要 】

Despite the fact that sleep deprivation substantially affects the way animals regulate their body temperature, the specific mechanisms behind this phenomenon are not well understood. In both mammals and flies, neural circuits regulating sleep and thermoregulation overlap, suggesting an interdependence that may be relevant for sleep function. To investigate this relationship further, we exposed flies to 12 h of sleep deprivation, or 48 h of sleep fragmentation and evaluated temperature preference in a thermal gradient. Flies exposed to 12 h of sleep deprivation chose warmer temperatures after sleep deprivation. Importantly, sleep fragmentation, which prevents flies from entering deeper stages of sleep, but does not activate sleep homeostatic mechanisms nor induce impairments in short-term memory also resulted in flies choosing warmer temperatures. To identify the underlying neuronal circuits, we used RNAi to knock down the receptor for Pigment dispersing factor, a peptide that influences circadian rhythms, temperature preference and sleep. Expressing UAS-PdfrRNAi in subsets of clock neurons prevented sleep fragmentation from increasing temperature preference. Finally, we evaluated temperature preference after flies had undergone a social jet lag protocol which is known to disrupt clock neurons. In this protocol, flies experience a 3 h light phase delay on Friday followed by a 3 h light advance on Sunday evening. Flies exposed to social jet lag exhibited an increase in temperature preference which persisted for several days. Our findings identify specific clock neurons that are modulated by sleep disruption to increase temperature preference. Moreover, our data indicate that temperature preference may be a more sensitive indicator of sleep disruption than learning and memory.

【 授权许可】

Unknown   
Copyright © 2023 Roach, Ford, Simhambhatla, Loutrianakis, Farah, Li, Periandri, Abdalla, Huang, Kalra and Shaw.