Staphylococcus aureus coa gene sequence analysis can prevent misidentification of coagulase-negative strains and contribute to their control in dairy cow herds

Frontiers in Microbiology

Microbiology

Clara Locatelli¹  Valentina Monistero¹  Maria Filippa Addis¹  Paolo Moroni²  Paola Cremonesi³  Bianca Castiglioni³  Stefano Gattolin³ 

[1] Department of Veterinary Medicine and Animal Sciences, Università degli Studi di Milano, Lodi, Italy;Laboratorio di Malattie Infettive degli Animali, Università degli Studi di Milano, Lodi, Italy;Department of Veterinary Medicine and Animal Sciences, Università degli Studi di Milano, Lodi, Italy;Laboratorio di Malattie Infettive degli Animali, Università degli Studi di Milano, Lodi, Italy;Quality Milk Production Services, Animal Health Diagnostic Center, Cornell University, Ithaca, NY, United States;Italian National Research Council, Institute of Agricultural Biology and Biotechnology, Lodi, Italy;

关键词: coagulase gene;    dairy cow;    genotyping;    sequencing;    Staphylococcus aureus;   

DOI  :  10.3389/fmicb.2023.1120305

 received in 2022-12-11, accepted in 2023-04-24,  发布年份 2023

来源: Frontiers

【 摘 要 】

Accurate and precise differentiation of staphylococci isolated from milk is of importance for udder health management. In particular, the rapid and specific identification of Staphylococcus aureus plays an essential role in the prevention and treatment programs for bovine mastitis. Plasma gelatinization in coagulase assays is routinely used to discriminate S. aureus from other species by detecting the presence of extracellular free staphylocoagulase. However, rarely occurring coagulase-deficient S. aureus strains can be responsible for clinical and subclinical mastitis cases. By investigating S. aureus isolates from a single herd over a 10-year period we identified the persistence of a phenotypically coagulase-negative S. aureus strain and pinpointed the possible cause to a single base pair deletion in the coa gene sequence. Our results support the need to integrate primary biochemical tests with molecular/sequence analysis approaches for correctly identifying and discriminating atypical S. aureus in bovine herds, as the coagulase test alone may fail to detect persistent mastitis-causing strains.

【 授权许可】

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Copyright © 2023 Locatelli, Gattolin, Monistero, Castiglioni, Moroni, Addis and Cremonesi.