Identification of serum biomarkers for necrotizing enterocolitis using aptamer-based proteomics

Frontiers in Pediatrics

Pediatrics

Lauren C. Frazer¹  Dhirendra K. Singh¹  Grace K. Bailey¹  Misty Good¹  Stephen Mackay¹  Claire M. Miller¹  Qingqing Gong²  Olivia N. Dewitt² 

[1]Division of Neonatal-Perinatal Medicine, Department of Pediatrics, The University of North Carolina at Chapel Hill, NC, United States

[2]Division of Newborn Medicine, Department of Pediatrics, Washington University School of Medicine, St. Louis, MO, United States

关键词: necrotizing enterocolitis;    prematurity;    aptamer;    SomaScan;    serum;    biomarker;   

DOI  :  10.3389/fped.2023.1184940

 received in 2023-03-12, accepted in 2023-05-10,  发布年份 2023

来源: Frontiers

【 摘 要 】

IntroductionNecrotizing enterocolitis (NEC) is a potentially fatal intestinal disease primarily affecting preterm infants. Early diagnosis of neonates with NEC is crucial to improving outcomes; however, traditional diagnostic tools remain inadequate. Biomarkers represent an opportunity to improve the speed and accuracy of diagnosis, but they are not routinely used in clinical practice.MethodsIn this study, we utilized an aptamer-based proteomic discovery assay to identify new serum biomarkers of NEC. We compared levels of serum proteins in neonates with and without NEC and identified ten differentially expressed serum proteins between these groups.ResultsWe detected two proteins, C-C motif chemokine ligand 16 (CCL16) and immunoglobulin heavy constant alpha 1 and 2 heterodimer (IGHA1 IGHA2), that were significantly increased during NEC and eight that were significantly decreased. Generation of receiver operating characteristic (ROC) curves revealed that alpha-fetoprotein (AUC = 0.926), glucagon (AUC = 0.860), and IGHA1 IGHA2 (AUC = 0.826) were the proteins that best differentiated patients with and without NEC.DiscussionThese findings indicate that further investigation into these serum proteins as a biomarker for NEC is warranted. In the future, laboratory tests incorporating these differentially expressed proteins may improve the ability of clinicians to diagnose infants with NEC rapidly and accurately.

【 授权许可】

Unknown   
© 2023 Mackay, Frazer, Bailey, Miller, Gong, DeWitt, Singh and Good.