2bRAD-M reveals the difference in microbial distribution between cancerous and benign ovarian tissues

Frontiers in Microbiology

Microbiology

Shuli Zhao¹  Tingting Wang²  Sunan Shen²  Shizhen Zhou²  Xiang Chen²  Xiaogang Wang²  Yaojun Zheng²  Chen Peng² 

[1] General Clinical Research Center, Nanjing First Hospital, Nanjing Medical University, Nanjing, China;The State Key Laboratory of Pharmaceutical Biotechnology, Division of Immunology, Medical School, Nanjing University, Nanjing, China;Jiangsu Key Laboratory of Molecular Medicine, Division of Immunology, Medical School, Nanjing University, Nanjing, China;

关键词: ovarian cancer;    microbiome;    tumor microbial;    2bRAD-M;    bacteria;   

DOI  :  10.3389/fmicb.2023.1231354

 received in 2023-05-31, accepted in 2023-08-09,  发布年份 2023

来源: Frontiers

【 摘 要 】

The development of ovarian cancer is closely related to various factors, such as environmental, genetic and microbiological factors. In previous research, bacteria were identified in human tumors by 16S rRNA sequencing. However, the microbial biomass in tumor tissue is too low and cannot be accurately identified by 16S rRNA sequencing. In our study, we employ 2bRAD sequencing for Microbiome (2bRAD-M), a new sequencing technology capable of accurately characterizing the low biomass microbiome (bacteria, fungi and archaea) at species resolution. Here we surveyed 20 ovarian samples, including 10 ovarian cancer samples and 10 benign ovarian samples. The sequencing results showed that a total of 373 microbial species were identified in both two groups, of which 90 species shared in the two groups. The Meta statistic indicated that Chlamydophila_abortus and CAG-873_sp900550395 were increased in the ovarian cancer tissues, while Lawsonella_clevelandensis_A, Ralstonia_sp001078575, Brevundimonas_aurantiaca, Ralstonia_sp900115545, Ralstonia_pickettii, Corynebacterium_kefirresidentii, Corynebacterium_sp000478175, Brevibacillus_D_fluminis, Ralstonia_sp000620465, and Ralstonia_mannitolilytica were more abundant in the benign ovarian tissues. This is the first use of 2bRAD-M technique to provide an important hint for better understanding of the ovarian cancer microbiome.

【 授权许可】

Unknown   
Copyright © 2023 Wang, Zheng, Chen, Peng, Zhou, Shen, Zhao and Wang.