Novel insights into genetic characteristics of blaGES-encoding plasmids from hospital sewage

Frontiers in Microbiology

Microbiology

Ryuji Koike¹  Yoko Nukui²  Yoshiaki Gu³  Isaac Prah⁴  Ryoichi Saito⁴  Yusuke Ota⁴  Samiratu Mahazu⁵ 

[1] Clinical Research Center, Tokyo Medical and Dental University Hospital, Tokyo, Japan;Department of Infection Control and Laboratory Medicine, Kyoto Prefectural University of Medicine, Kyoto, Japan;Department of Infectious Diseases, Tokyo Medical and Dental University, Tokyo, Japan;Department of Molecular Microbiology and Immunology, Tokyo Medical and Dental University, Tokyo, Japan;Department of Molecular Microbiology and Immunology, Tokyo Medical and Dental University, Tokyo, Japan;Department of Parasitology and Tropical Medicine, Tokyo Medical and Dental University, Tokyo, Japan;

关键词: GES;    hospital sewage;    plasmid;    integron;    toxin-antitoxin system;   

DOI  :  10.3389/fmicb.2023.1209195

 received in 2023-04-20, accepted in 2023-08-01,  发布年份 2023

来源: Frontiers

【 摘 要 】

IntroductionThe prevalence of Guiana extended-spectrum (GES)-type carbapenemase producers is increasing worldwide, and hospital water environments are considered as potential reservoirs. However, the genetic features underlying this resistance are not yet fully understood. This study aimed to characterize blaGES-encoding plasmids from a single-hospital sewage sample in Japan.MethodsCarbapenemase producers were screened using carbapenemase-selective agar and polymerase chain reaction. Whole-genome sequencing analyzes were performed on the carbapenemase-producing isolates.ResultsEleven gram-negative bacteria (four Enterobacter spp., three Klebsiella spp., three Aeromonas spp., and one Serratia spp.) with blaGES-24 (n = 6), blaGES-6 (n = 4), and blaGES-5 (n = 1) were isolated from the sewage sample. Five blaGES-24 and a blaGES-5 were localized in IncP-6 plasmids, whereas three blaGES-6 plasmids were localized in IncC plasmids with IncF-like regions. The remaining blaGES-6 and blaGES-24 were, respectively, localized on IncFIB-containing plasmids with IncF-like regions and a plasmid with an IncW-like replication protein. The IncP-6 and IncW-like plasmids had a close genetic relationship with plasmids from Japan, whereas the IncC/IncF-like and IncFIB/IncF-like plasmids were closely related to those from the United States and Europe. All blaGES genes were located on the class 1 integron cassette of the Tn3 transposon-related region, and the IncC/IncF-like plasmid carried two copies of the integron cassette. Eight of the eleven blaGES-encoding plasmids contained toxin-antitoxin system genes.DiscussionThe findings on the plasmids and the novel genetic content from a single wastewater sample extend our understanding regarding the diversity of resistance and the associated spread of blaGES, suggesting their high adaptability to hospital effluents. These findings highlight the need for the continuous monitoring of environmental GES-type carbapenemase producers to control their dissemination.

【 授权许可】

Unknown   
Copyright © 2023 Ota, Prah, Mahazu, Gu, Nukui, Koike and Saito.