Core microbiota of wheat rhizosphere under Upper Indo-Gangetic plains and their response to soil physicochemical properties

Frontiers in Plant Science

Plant Science

Arjun Singh¹  Sudhir Srivastava²  Mohammad Samir Farooqi²  Anu Sharma²  Hillol Chakdar³  Mohammad Tarique Zeyad³  Adarsh Kumar³  Waquar Akhter Ansari³  Alok Kumar Srivastava³  Murugan Kumar³ 

[1] ICAR-Central Soil Salinity Research Institute, Regional Research Station (RRS), Lucknow, Uttar Pradesh, India;ICAR-Indian Agricultural Statistics Research Institute, New Delhi, India;ICAR-National Bureau of Agriculturally Important Microorganisms, Mau, Uttar Pradesh, India;

关键词: core microbiota;    Indo-Gangetitc plains;    keystone taxa;    rhizosphere;    wheat;   

DOI  :  10.3389/fpls.2023.1186162

 received in 2023-03-14, accepted in 2023-04-21,  发布年份 2023

来源: Frontiers

【 摘 要 】

Wheat is widely cultivated in the Indo-Gangetic plains of India and forms the major staple food in the region. Understanding microbial community structure in wheat rhizosphere along the Indo-Gangetic plain and their association with soil properties can be an important base for developing strategies for microbial formulations. In the present study, an attempt was made to identify the core microbiota of wheat rhizosphere through a culture-independent approach. Rhizospheric soil samples were collected from 20 different sites along the upper Indo-Gangetic plains and their bacterial community composition was analyzed based on sequencing of the V3-V4 region of the 16S rRNA gene. Diversity analysis has shown significant variation in bacterial diversity among the sites. The taxonomic profile identified Proteobacteria, Chloroflexi, Actinobacteria, Bacteroidetes, Acidobacteria, Gemmatimonadetes, Planctomycetes, Verrucomicrobia, Firmicutes, and Cyanobacteria as the most dominant phyla in the wheat rhizosphere in the region. Core microbiota analysis revealed 188 taxa as core microbiota of wheat rhizosphere with eight genera recording more than 0.5% relative abundance. The order of most abundant genera in the core microbiota is Roseiflexus> Flavobacterium> Gemmatimonas> Haliangium> Iamia> Flavisolibacter> Ohtaekwangia> Herpetosiphon. Flavobacterium, Thermomonas, Massilia, Unclassified Rhizobiaceae, and Unclassified Crenarchaeota were identified as keystone taxa of the wheat rhizosphere. Correlation studies revealed, pH, organic carbon content, and contents of available nitrogen, phosphorus, and iron as the major factors driving bacterial diversity in the wheat rhizosphere. Redundancy analysis has shown the impact of different soil properties on the relative abundance of different genera of the core microbiota. The results of the present study can be used as a prelude to be developing microbial formulations based on core microbiota.

【 授权许可】

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Copyright © 2023 Kumar, Ansari, Zeyad, Singh, Chakdar, Kumar, Farooqi, Sharma, Srivastava and Srivastava