Dissection of the catalytic and regulatory structure-function relationships of Csk protein tyrosine kinase

Frontiers in Cell and Developmental Biology

Cell and Developmental Biology

Marina K. Ayrapetov¹  Gongqin Sun² 

[1] Department of Cell and Molecular Biology, University of Rhode Island, Kingston, RI, United States;null;

关键词: Csk and Chk;    Src regulation;    substrate recognition;    kinase regulation;    structure-function relationships;    domain-domain interaction;    redox regulation;   

DOI  :  10.3389/fcell.2023.1148352

 received in 2023-01-20, accepted in 2023-02-21,  发布年份 2023

来源: Frontiers

【 摘 要 】

Protein tyrosine kinases (PTKs) are a large enzyme family that regulates many cellular processes. The key to their broad role in signaling is their tunable substrate specificity and regulatory mechanisms that allow each to respond to appropriate regulatory signals and phosphorylate the correct physiological protein substrates. Thus, in addition to the general PTK catalytic platform, each PTK acquires unique structural motifs that confer a unique combination of catalytic and regulatory properties. Understanding the structural basis for these properties is essential for understanding and manipulating the PTK-based signaling networks in normal and cancer cells. C-terminal Src kinase (Csk) and its homolog, Csk-homologous kinase (Chk), phosphorylate Src family kinases on a C-terminal Tyr residue and negatively regulate their kinase activity. While this regulatory function is biologically essential, Csk and Chk have also been excellent model PTKs for dissecting the structural basis of PTK catalysis and regulation. In this article, we review the structure-function studies of Csk and Chk that shed light on the regulatory and catalytic mechanisms of protein tyrosine kinases in general.

【 授权许可】

Unknown   
Copyright © 2023 Sun and Ayrapetov.