Characterizing conjugative plasmids from an antibiotic-resistant dataset for use as broad-host delivery vectors

Frontiers in Microbiology

Microbiology

Héctor G. Loyola Irizarry¹  Ilana L. Brito² 

[1] Meinig School of Biomedical Engineering, Cornell University, Ithaca, NY, United States;null;

关键词: plasmids;    conjugation;    broad host-range (BHR);    CDC & FDA AR Isolate Bank;    microbiome engineering;   

DOI  :  10.3389/fmicb.2023.1199640

 received in 2023-04-03, accepted in 2023-05-24,  发布年份 2023

来源: Frontiers

【 摘 要 】

Human microbiome engineering is increasingly proposed as a way to modulate health outcomes. However, one of the current limitations to engineering microbial communities in situ is delivery of a genetic payload for introducing or modifying genes. Indeed, there is a need to identify novel broad-host delivery vectors for microbiome engineering. Therefore, in this study, we characterized conjugative plasmids from a publicly available dataset of antibiotic-resistant isolate genomes in order to identify potential broad-host vectors for further applications. From the 199 closed genomes available in the CDC & FDA AR Isolate Bank, we identified 439 plasmids, of which 126 were predicted to be mobilizable and 206 conjugative. Various characteristics of the conjugative plasmids, such as size, replication origin, conjugation machinery, host defense mechanisms, and plasmid stability proteins, were analyzed to determine these plasmids’ potential host-range. Following this analysis, we clustered plasmid sequences and chose 22 unique, broad-host range plasmids that would be suitable for use as delivery vectors. This novel set of plasmids will provide a valuable resource for engineering microbial communities.

【 授权许可】

Unknown   
Copyright © 2023 Loyola Irizarry and Brito.