Knockdown of Long Non-Coding RNA KCNQ1OT1 Restrained Glioma Cells’ Malignancy by Activating miR-370/CCNE2 Axis

Frontiers in Cellular Neuroscience

Neuroscience

Jun Ma¹  Yixue Xue¹  Junqing Guo¹  Wei Gong¹  Yana Gao¹  Zhiqing Li¹  Wei Tao¹  Jiajia Chen¹  Xiaobai Liu²  Yunhui Liu²  Jian Zheng² 

[1] Department of Neurobiology, College of Basic Medicine, China Medical University, Shenyang, China;Key Laboratory of Cell Biology, Ministry of Public Health of China, Key Laboratory of Medical Cell Biology, Ministry of Education of China, China Medical University, Shenyang, China;Department of Neurosurgery, Shengjing Hospital of China Medical University, Shenyang, China;Liaoning Research Center for Translational Medicine in Nervous System Disease, Shenyang, China;

关键词: glioma;    KCNQ1OT1;    miR-370;    CCNE2;    hippo pathway;   

DOI  :  10.3389/fncel.2017.00084

 received in 2016-11-14, accepted in 2017-03-10,  发布年份 2017

来源: Frontiers

【 摘 要 】

Accumulating evidence has highlighted the potential role of long non-coding RNAs (lncRNAs) as biomarkers and therapeutic targets in solid tumors. Here, we elucidated the function and possible molecular mechanisms of lncRNA KCNQ1OT1 in human glioma U87 and U251 cells. Quantitative Real-Time polymerase chain reaction (qRT-PCR) demonstrated that KCNQ1OT1 expression was up-regulated in glioma tissues and cells. Knockdown of KCNQ1OT1 exerted tumor-suppressive function in glioma cells. Moreover, a binding region was confirmed between KCNQ1OT1 and miR-370 by dual-luciferase assays. qRT-PCR showed that miR-370 was down-regulated in human glioma tissue and cells. In addition, restoration of miR-370 exerted tumor-suppressive function via inhibiting cell proliferation, migration and invasion, while promoting the apoptosis of human glioma cells. Knockdown of KCNQ1OT1 decreased the expression level of Cyclin E2 (CCNE2) by binding to miR-370. Further, miR-370 bound to CCNE2 3′UTR region and decreased the expression of CCNE2. These results provided a comprehensive analysis of KCNQ1OT1-miR-370-CCNE2 axis in human glioma cells and might provide a novel strategy for glioma treatment.

【 授权许可】

Unknown   
Copyright © 2017 Gong, Zheng, Liu, Liu, Guo, Gao, Tao, Chen, Li, Ma and Xue.