Circulating bile acid profile characteristics in PCOS patients and the role of bile acids in predicting the pathogenesis of PCOS

Frontiers in Endocrinology

Endocrinology

Wei Liu¹  Siyu Lin¹  Tao Tao¹  Yushan Li¹  Jie Yu¹  Yuchen Zhu¹  Yi Zhang¹ 

[1] Department of Endocrinology and Metabolism, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China;

关键词: bile acid;    OPLS-DA model;    PCOS;    biomarker;    HPLC/MS;   

DOI  :  10.3389/fendo.2023.1239276

 received in 2023-06-13, accepted in 2023-08-09,  发布年份 2023

来源: Frontiers

【 摘 要 】

BackgroundThe metabolic profile of bile acids and their potential role as biomarkers in the pathogenesis of polycystic ovary syndrome (PCOS) have not been thoroughly characterized. Assessing their predictive value for PCOS is of significant importance.MethodsIn this study, we enrolled 408 women with PCOS and 204 non-PCOS controls. The serum bile acid profile was measured using high-performance liquid chromatography-tandem mass spectrometry (LC/MS). We analyzed the differences in serum bile acid profiles between PCOS patients using the OPLS-DA model. Additionally, we examined the relationship between bile acid profiles and parameters related to glucose metabolism and hyperandrogenism. ROC analysis was employed to identify potential biomarkers for PCOS pathogenesis. XGboost was utilized for cross-validation.ResultsThe bile acid profile was found to be altered in PCOS patients. Specifically, the primary and secondary unconjugated bile acid fractions were significantly higher in the PCOS population. We identified five bile acid metabolite candidates that exhibited the most significant differences between PCOS and non-PCOS controls. DCA was associated with deposition index, fasting and postprandial insulin but was influenced by testosterone. CDCA and LCA combined with testosterone showed potential as biomarkers for the pathogenesis of PCOS.ConclusionThe circulating bile acid profile undergoes changes in PCOS. DCA is associated with deposition index, fasting and postprandial insulin and its level is influenced by testosterone. CDCA and LCA combined with testosterone have the potential to serve as biomarkers for the pathogenesis of PCOS.

【 授权许可】

Unknown   
Copyright © 2023 Yu, Zhang, Zhu, Li, Lin, Liu and Tao