Mapping metabolite change in the mouse brain after esketamine injection by ambient mass spectrometry imaging and metabolomics

Frontiers in Psychiatry

Psychiatry

Kai Wu¹  Ze-Lin Li²  Qian Wang²  Yan-Lin Zhou²  Zheng-Yi Luo²  Guan-Xi Liu³  Yu-Ping Ning³  Su-Yan Lin⁴ 

[1] School of Biomedical Sciences and Engineering, South China University of Technology, Guangzhou International Campus, Guangzhou, China;The Affiliated Brain Hospital of Guangzhou Medical University, Guangzhou Huiai Hospital, Guangzhou, China;The First School of Clinical Medicine, Southern Medical University, Guangzhou, China;The Affiliated Brain Hospital of Guangzhou Medical University, Guangzhou Huiai Hospital, Guangzhou, China;The Second Affiliated Hospital of Guangzhou Medical University, Guangzhou, China;

关键词: ketamine;    depression;    antidepressant;    spatial metabolomics;    mass spectrometry imaging;   

DOI  :  10.3389/fpsyt.2023.1109344

 received in 2022-11-27, accepted in 2023-03-20,  发布年份 2023

来源: Frontiers

【 摘 要 】

Ketamine is a new, fast, and effective antidepression treatment method; however, the possible dissociation effects, sensory changes, abuse risk, and the inability to accurately identify whether patients have a significant response to ketamine limit its clinical use. Further exploration of the antidepressant mechanisms of ketamine will contribute to its safe and practical application. Metabolites, the products of upstream gene expression and protein regulatory networks, play an essential role in various physiological and pathophysiological processes. In traditional metabonomics it is difficult to achieve the spatial localization of metabolites, which limits the further analysis of brain metabonomics by researchers. Here, we used a metabolic network mapping method called ambient air flow-assisted desorption electrospray ionization (AFADESI)-mass spectrometry imaging (MSI). We found the main changes in glycerophospholipid metabolism around the brain and sphingolipid metabolism changed mainly in the globus pallidus, which showed the most significant metabolite change after esketamine injection. The spatial distribution of metabolic changes was evaluated in the whole brain, and the potential mechanism of esketamine’s antidepressant effect was explored in this research.

【 授权许可】

Unknown   
Copyright © 2023 Liu, Li, Lin, Wang, Luo, Wu, Zhou and Ning.