Bacterial microbiome variation across symbiotic states and clonal lines in a cnidarian model

Frontiers in Marine Science

Marine Science

Ezra Curtis¹  Riccardo Racicot¹  Joanne Moseley¹  Rachel M. Wright² 

[1] Department of Biological Sciences, Smith College, Northampton, MA, United States;Department of Biological Sciences, Smith College, Northampton, MA, United States;Department of Biological Sciences, Southern Methodist University, Dallas, TX, United States;

关键词: Aiptasia;    Exaiptasia;    symbiosis;    microbial community;    coral reef;   

DOI  :  10.3389/fmars.2023.1113043

 received in 2022-12-01, accepted in 2023-01-31,  发布年份 2023

来源: Frontiers

【 摘 要 】

IntroductionExaiptasia diaphana is a popular model organism for exploring the symbiotic relationship observed between cnidarians and their microsymbionts. While physiological roles of algal photosymbionts (Symbiodinaceae) are well studied, the contributions of bacterial communities are less defined in this system.MethodsWe investigated microbial variation between distinct parts of the body and symbiotic state across four genets held in identical environmental conditions using 16s rRNA gene amplicon sequencing.ResultsWe found differentially abundant taxa between body part and symbiotic state that highlight the roles these bacteria may play in holobiont heterotrophy and nutrient cycling. Beta-diversity analysis revealed distinct communities between symbiotic states consistent with previous studies; however, we did not observe the presence of previously reported core microbiota. We also found community differences across clonal lines, despite years of identical rearing conditions. ConclusionThese findings suggest the Exaiptasia bacterial microbiome is greatly influenced by host genetics and unpredictable environmental influences.

【 授权许可】

Unknown   
Copyright © 2023 Curtis, Moseley, Racicot and Wright