Frontiers in Molecular Biosciences | |
Anti-schistosomal immunity to core xylose/fucose in N-glycans | |
Molecular Biosciences | |
Nina Salinger Prasanphanich1  Kristoffer Leon1  Richard D. Cummings2  Jamie Heimburg-Molinaro2  Charles B. Shoemaker3  W. Evan Secor4  | |
[1] Department of Biochemistry, Emory University School of Medicine, Atlanta, GA, United States;Department of Biochemistry, Emory University School of Medicine, Atlanta, GA, United States;National Center for Functional Glycomics, Department of Surgery, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA, United States;Department of Infectious Disease and Global Health, Tufts University Cummings School of Veterinary Medicine, North Grafton, MA, United States;Division of Parasitic Diseases and Malaria, Centers for Disease Control and Prevention, Atlanta, GA, United States; | |
关键词: schistosomiasis; immunity; N-glycans; antigens; core fucose; core xylose; | |
DOI : 10.3389/fmolb.2023.1142620 | |
received in 2023-01-11, accepted in 2023-03-20, 发布年份 2023 | |
来源: Frontiers | |
【 摘 要 】
Schistosomiasis is a globally prevalent, debilitating disease that is poorly controlled by chemotherapy and for which no vaccine exists. While partial resistance in people may develop over time with repeated infections and treatments, some animals, including the brown rat (Rattus norvegicus), are only semi-permissive and have natural protection. To understand the basis of this protection, we explored the nature of the immune response in the brown rat to infection by Schistosoma mansoni. Infection leads to production of IgG to parasite glycoproteins with complex-type N-glycans that contain a non-mammalian-type modification by core α2-Xylose and core α3-Fucose (core Xyl/Fuc). These epitopes are expressed on the surfaces of schistosomula and adult worms. Importantly, IgG to these epitopes can kill schistosomula by a complement-dependent process in vitro. Additionally, sera from both infected rhesus monkey and infected brown rat were capable of killing schistosomula in a manner inhibited by glycopeptides containing core Xyl/Fuc. These results demonstrate that protective antibodies to schistosome infections in brown rats and rhesus monkeys include IgG responses to the core Xyl/Fuc epitopes in surface-expressed N-glycans, and raise the potential of novel glyco-based vaccines that might be developed to combat this disease.
【 授权许可】
Unknown
Copyright © 2023 Prasanphanich, Leon, Secor, Shoemaker, Heimburg-Molinaro and Cummings.
【 预 览 】
Files | Size | Format | View |
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RO202310104051126ZK.pdf | 1841KB | download |