Transcriptomic and functional analyses reveal the molecular mechanisms underlying Fe-mediated tobacco resistance to potato virus Y infection

Frontiers in Plant Science

Plant Science

Qing Mu¹  Di Zhu¹  Zhiping Wang²  Xinyu Lan²  Huiyan Guo²  Mengnan An²  Rui Li²  Zihao Xia²  Yuanhua Wu²  Chuantao Xu³  Qiang Xie⁴  Yonghui Zhang⁴ 

[1] Guizhou Qianxinan Prefectural Tobacco Company, Xingyi, China;Liaoning Key Laboratory of Plant Pathology, College of Plant Protection, Shenyang Agricultural University, Shenyang, China;Liaoning Key Laboratory of Plant Pathology, College of Plant Protection, Shenyang Agricultural University, Shenyang, China;Luzhou City Company of Sichuan Province Tobacco Company, Luzhou, China;Luzhou City Company of Sichuan Province Tobacco Company, Luzhou, China;

关键词: PVY;    Fe;    full-length transcriptome;    Illumina RNA sequencing;    virus-induced gene silencing;   

DOI  :  10.3389/fpls.2023.1163679

 received in 2023-02-11, accepted in 2023-03-15,  发布年份 2023

来源: Frontiers

【 摘 要 】

Potato virus Y (PVY) mainly infects Solanaceous crops, resulting in considerable losses in the yield and quality. Iron (Fe) is involved in various biological processes in plants, but its roles in resistance to PVY infection has not been reported. In this study, foliar application of Fe could effectively inhibit early infection of PVY, and a full-length transcriptome and Illumina RNA sequencing was performed to investigate its modes of action in PVY-infected Nicotiana tabacum. The results showed that 18,074 alternative splicing variants, 3,654 fusion transcripts, 3,086 long non-coding RNAs and 14,403 differentially expressed genes (DEGs) were identified. Specifically, Fe application down-regulated the expression levels of the DEGs related to phospholipid hydrolysis, phospholipid signal, cell wall biosynthesis, transcription factors (TFs) and photosystem I composition, while those involved with photosynthetic electron transport chain (PETC) were up-regulated at 1 day post inoculation (dpi). At 3 dpi, these DEGs related to photosystem II composition, PETC, molecular chaperones, protein degradation and some TFs were up-regulated, while those associated with light-harvesting, phospholipid hydrolysis, cell wall biosynthesis were down-regulated. At 9 dpi, Fe application had little effects on resistance to PVY infection and transcript profiles. Functional analysis of these potentially critical DEGs was thereafter performed using virus-induced gene silencing approaches and the results showed that NbCat-6A positively regulates PVY infection, while the reduced expressions of NbWRKY26, NbnsLTP, NbFAD3 and NbHSP90 significantly promote PVY infection in N. benthamiana. Our results elucidated the regulatory network of Fe-mediated resistance to PVY infection in plants, and the functional candidate genes also provide important theoretical bases to further improve host resistance against PVY infection.

【 授权许可】

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Copyright © 2023 Xu, Guo, Li, Lan, Zhang, Xie, Zhu, Mu, Wang, An, Xia and Wu