3D-bioprinting of patient-derived cardiac tissue models for studying congenital heart disease

Frontiers in Cardiovascular Medicine

Cardiovascular Medicine

Jayne T. Wolfe¹  Wei He¹  Akankshya Shradhanjali¹  Hilda Jurkiewicz¹  Brandon J. Tefft²  John F. LaDisa³  Aoy Tomita-Mitchell⁴  Huan-Ling Liang⁵  Min-Su Kim⁵  Michael E. Mitchell⁶  Bonnie P. Freudinger⁷  Lobat Tayebi⁸  Andrew S. Greene⁹ 

[1] Department of Biomedical Engineering, Medical College of Wisconsin & Marquette University, Milwaukee, WI, United States;Department of Biomedical Engineering, Medical College of Wisconsin & Marquette University, Milwaukee, WI, United States;Cardiovascular Center, Medical College of Wisconsin, Milwaukee, WI, United States;Department of Biomedical Engineering, Medical College of Wisconsin & Marquette University, Milwaukee, WI, United States;Department of Pediatrics - Section of Cardiology, Children’s Wisconsin, Milwaukee, WI, United States;The Herma Heart Institute, Children’s Wisconsin, Milwaukee, WI, United States;Cardiovascular Center, Medical College of Wisconsin, Milwaukee, WI, United States;Department of Biomedical Engineering, Medical College of Wisconsin & Marquette University, Milwaukee, WI, United States;Department of Surgery, Medical College of Wisconsin, Milwaukee, WI, United States;The Herma Heart Institute, Children’s Wisconsin, Milwaukee, WI, United States;Cardiovascular Center, Medical College of Wisconsin, Milwaukee, WI, United States;Department of Surgery, Medical College of Wisconsin, Milwaukee, WI, United States;Department of Surgery, Medical College of Wisconsin, Milwaukee, WI, United States;The Herma Heart Institute, Children’s Wisconsin, Milwaukee, WI, United States;Engineering Core, Medical College of Wisconsin, Milwaukee, WI, United States;School of Dentistry, Marquette University, Milwaukee, WI, United States;The Jackson Laboratory, Bar Harbor, ME, United States;

关键词: 3D-bioprinting;    hydrogel;    induced pluripotent stem cell;    cardiomyocyte;    congenital heart disease;    hypoplastic left heart syndrome;   

DOI  :  10.3389/fcvm.2023.1162731

 received in 2023-02-09, accepted in 2023-04-27,  发布年份 2023

来源: Frontiers

【 摘 要 】

IntroductionCongenital heart disease is the leading cause of death related to birth defects and affects 1 out of every 100 live births. Induced pluripotent stem cell technology has allowed for patient-derived cardiomyocytes to be studied in vitro. An approach to bioengineer these cells into a physiologically accurate cardiac tissue model is needed in order to study the disease and evaluate potential treatment strategies.MethodsTo accomplish this, we have developed a protocol to 3D-bioprint cardiac tissue constructs comprised of patient-derived cardiomyocytes within a hydrogel bioink based on laminin-521.ResultsCardiomyocytes remained viable and demonstrated appropriate phenotype and function including spontaneous contraction. Contraction remained consistent during 30 days of culture based on displacement measurements. Furthermore, tissue constructs demonstrated progressive maturation based on sarcomere structure and gene expression analysis. Gene expression analysis also revealed enhanced maturation in 3D constructs compared to 2D cell culture.DiscussionThis combination of patient-derived cardiomyocytes and 3D-bioprinting represents a promising platform for studying congenital heart disease and evaluating individualized treatment strategies.

【 授权许可】

Unknown   
© 2023 Wolfe, He, Kim, Liang, Shradhanjali, Jurkiewicz, Freudinger, Greene, LaDisa, Tayebi, Mitchell, Tomita-Mitchell and Tefft.