Isolation of archaeal viruses with lipid membrane from Tengchong acidic hot springs

Frontiers in Microbiology

Microbiology

Changyi Zhang¹  Xi Feng²  Wei Yang²  Xinyu Liu²  Yanan Li²  Chang Tian²  Zhirui Zeng³ 

[1] Carl R. Woese Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, IL, United States;Department of Ocean Science and Engineering, Southern University of Science and Technology, Shenzhen, China;Department of Ocean Science and Engineering, Southern University of Science and Technology, Shenzhen, China;Southern Marine Science and Engineering Guangdong Laboratory, Guangzhou, China;

关键词: archaeal virus;    Sulfolobaceae;    hot springs;    Tengchong;    viral membrane lipid;   

DOI  :  10.3389/fmicb.2023.1134935

 received in 2022-12-31, accepted in 2023-03-14,  发布年份 2023

来源: Frontiers

【 摘 要 】

Archaeal viruses are one of the most mysterious parts of the virosphere because of their diverse morphologies and unique genome contents. The crenarchaeal viruses are commonly found in high temperature and acidic hot springs, and the number of identified crenarchaeal viruses is being rapidly increased in recent two decades. Over fifty viruses infecting the members of the order Sulfolobales have been identified, most of which are from hot springs distributed in the United States, Russia, Iceland, Japan, and Italy. To further expand the reservoir of viruses infecting strains of Sulfolobaceae, we investigated virus diversity through cultivation-dependent approaches in hot springs in Tengchong, Yunnan, China. Eight different virus-like particles were detected in enrichment cultures, among which five new archaeal viruses were isolated and characterized. We showed that these viruses can infect acidophilic hyperthermophiles belonging to three different genera of the family Sulfolobaceae, namely, Saccharolobus, Sulfolobus, and Metallosphaera. We also compared the lipid compositions of the viral and cellular membranes and found that the lipid composition of some viral envelopes was very different from that of the host membrane. Collectively, our results showed that the Tengchong hot springs harbor highly diverse viruses, providing excellent models for archaeal virus-host studies.

【 授权许可】

Unknown   
Copyright © 2023 Feng, Li, Tian, Yang, Liu, Zhang and Zeng.