Effect of gut microbiome regulated Taohong Siwu Decoction metabolism on glioma cell phenotype

Frontiers in Cellular and Infection Microbiology

Cellular and Infection Microbiology

Weijiang Wu¹  Hua Lu¹  Suyin Feng²  Quan Wan³  Chenyang Zhang⁴  Xiaojie Lu⁵ 

[1] Department of Neurosurgery, Affiliated Hospital of Jiangnan University, Wuxi, China;Department of Neurosurgery, Affiliated Hospital of Jiangnan University, Wuxi, China;Neuroscience Center, Wuxi School of Medicine, Jiangnan University, Wuxi, China;Jiangnan University Medical Center, Wuxi, China;Neuroscience Center, Wuxi School of Medicine, Jiangnan University, Wuxi, China;Institute of Integrative Chinese and Western Medicine, Affiliated Hospital of Jiangnan University, Wuxi, China;Neuroscience Center, Wuxi School of Medicine, Jiangnan University, Wuxi, China;Jiangnan University Medical Center, Wuxi, China;Wuxi Neurological Institute, Wuxi No.2 People’s Hospital, Wuxi, China;

关键词: Taohong Siwu Decoction;    intestinal flora;    CDC6 pathway;    glioma cells;    RNA-seq analysis;   

DOI  :  10.3389/fcimb.2023.1192589

 received in 2023-03-23, accepted in 2023-05-16,  发布年份 2023

来源: Frontiers

【 摘 要 】

IntroductionTo establish a new model for exploring the mechanism of the gut microbiome and drug metabolism, we explored whether Taohong Siwu Decoction acts after metabolism by intestinal flora under the premise of clarifying the interaction between intestinal flora and drug metabolism.MethodsTaohong Siwu Decoction (TSD) was fed to germ-free mice and conventional mice, respectively. The serum from both groups of mice was removed and co-cultured with glioma cells in vitro. The co-cultured glioma cells were compared separately for changes at the RNA level using RNA-seq technology. The genes of interest in the comparison results were selected for validation.ResultsThe differences in the phenotypic alterations of glioma cells between serum from TSD-fed germ-free mice and normal mice were statistically significant. In vitro experiments showed that Taohong Siwu Decoction-fed normal mouse serum-stimulated glioma cells, which inhibited proliferation and increased autophagy. RNA-seq analysis showed that TSD-fed normal mouse serum could regulate CDC6 pathway activity in glioma cells. The therapeutic effect of TSD is significantly influenced by intestinal flora.ConclusionThe treatment of tumors by TSD may be modulated by intestinal flora. We established a new method to quantify the relationship between intestinal flora and the regulation of TSD efficacy through this study.

【 授权许可】

Unknown   
Copyright © 2023 Feng, Wan, Wu, Zhang, Lu and Lu