Molecular mapping of quantitative trait loci for resistance to early blight in tomatoes

Frontiers in Plant Science

Plant Science

Sung-Chur Sim¹  Tika B. Adhikari²  Frank J. Louws³  Muhammad Irfan Siddique⁴  Dilip R. Panthee⁴ 

[1] Department of Bioresources Engineering, Sejong University, Seoul, Republic of Korea;Department of Entomology and Plant Pathology, North Carolina State University, Raleigh, NC, United States;Department of Entomology and Plant Pathology, North Carolina State University, Raleigh, NC, United States;Department of Horticultural Science, North Carolina State University, Raleigh, NC, United States;Department of Horticultural Science, North Carolina State University, Mountain Horticultural Crops Research and Extension Center, Mills River, NC, United States;

关键词: early blight;    heritability estimates;    QTL analysis;    tomatoes;    Solanum lycopersicum;   

DOI  :  10.3389/fpls.2023.1135884

 received in 2023-01-02, accepted in 2023-05-02,  发布年份 2023

来源: Frontiers

【 摘 要 】

Early blight (EB), caused by Alternaria linariae (Neerg.) (syn. A. tomatophila) Simmons, is a disease that affects tomatoes (Solanum lycopersicum L.) throughout the world, with tremendous economic implications. The objective of the present study was to map the quantitative trait loci (QTL) associated with EB resistance in tomatoes. The F2 and F2:3 mapping populations consisting of 174 lines derived from NC 1CELBR (resistant) × Fla. 7775 (susceptible) were evaluated under natural conditions in the field in 2011 and in the greenhouse in 2015 by artificial inoculation. In all, 375 Kompetitive Allele Specific PCR (KASP) assays were used for genotyping parents and the F2 population. The broad-sense heritability estimate for phenotypic data was 28.3%, and 25.3% for 2011, and 2015 disease evaluations, respectively. QTL analysis revealed six QTLs associated with EB resistance on chromosomes 2, 8, and 11 (LOD 4.0 to 9.1), explaining phenotypic variation ranging from 3.8 to 21.0%. These results demonstrate that genetic control of EB resistance in NC 1CELBR is polygenic. This study may facilitate further fine mapping of the EB-resistant QTL and marker-assisted selection (MAS) to transfer EB resistance genes into elite tomato varieties, including broadening the genetic diversity of EB resistance in tomatoes.

【 授权许可】

Unknown   
Copyright © 2023 Adhikari, Siddique, Louws, Sim and Panthee