Postoperative circulating tumor DNA testing based on tumor naïve strategy after liver metastasis surgery in colorectal cancer patients

Frontiers in Oncology

Oncology

Xinning Chen¹  Huiqin Jiang¹  Fei Huang¹  Yihui Yang¹  Minna Shen¹  Baishen Pan²  Chunyan Zhang³  Beili Wang⁴  Wei Guo⁵ 

[1] Department of Laboratory Medicine, Zhongshan Hospital, Fudan University, Shanghai, China;Department of Laboratory Medicine, Zhongshan Hospital, Fudan University, Shanghai, China;Department of Laboratory Medicine, Wusong Branch, Zhongshan Hospital, Fudan University, Shanghai, China;Department of Laboratory Medicine, Zhongshan Hospital, Fudan University, Shanghai, China;Department of Laboratory Medicine, Xiamen Branch, Zhongshan Hospital, Fudan University, Xiamen, China;Department of Laboratory Medicine, Zhongshan Hospital, Fudan University, Shanghai, China;Department of Laboratory Medicine, Xiamen Branch, Zhongshan Hospital, Fudan University, Xiamen, China;Department of Laboratory Medicine, Wusong Branch, Zhongshan Hospital, Fudan University, Shanghai, China;Department of Laboratory Medicine, Zhongshan Hospital, Fudan University, Shanghai, China;Department of Laboratory Medicine, Xiamen Branch, Zhongshan Hospital, Fudan University, Xiamen, China;Department of Laboratory Medicine, Wusong Branch, Zhongshan Hospital, Fudan University, Shanghai, China;Branch of National Clinical Research Center for Laboratory Medicine, Shanghai, China;Cancer Center, Zhongshan Hospital, Fudan University, Shanghai, China;

关键词: minimal residual disease (MRD);    circulating tumor DNA (ctDNA);    colorectal cancer liver metastases (CRLM);    next-generation sequencing – NGS;    recurrence;   

DOI  :  10.3389/fonc.2023.1153685

 received in 2023-01-29, accepted in 2023-04-11,  发布年份 2023

来源: Frontiers

【 摘 要 】

ObjectiveThere is still a lack of highly sensitive methods for monitoring recurrence of colorectal cancer patients after liver metastasis surgery. The aim of this study was to evaluate the prognostic value of tumor-naive ctDNA detection after resection of colorectal liver metastases (CRLM).MethodsPatients with resectable CRLM were prospectively enrolled. Based on the tumor-naive strategy, NGS panels containing 15 colorectal cancer hotspot mutated genes were used to detect ctDNA 3-6 weeks after surgery.ResultsA total of 67 patients were included in the study, and the positive rate of postoperative ctDNA was 77.6% (52/67). Patients with positive ctDNA had a significantly higher risk of recurrence after surgery (HR 3.596, 95% CI 1.479 to 8.744, P = 0.005), and a higher proportion relapsed within 3 months after surgery (46.7% vs 3.8%). The C-index of postoperative ctDNA in predicting recurrence was higher than that of CRS and postoperative CEA. The nomogram combining CRS and postoperative ctDNA can improve the accuracy of recurrence prediction.ConclusionTumor-naive ctDNA detection can detect molecular residual lesions in patients with colorectal cancer after liver metastasis, and its prognostic value is superior to conventional clinical factors.

【 授权许可】

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Copyright © 2023 Jiang, Huang, Yang, Chen, Shen, Zhang, Pan, Wang and Guo