Compartmentalization engineering of yeasts to overcome precursor limitations and cytotoxicity in terpenoid production

Frontiers in Bioengineering and Biotechnology

Bioengineering and Biotechnology

Lifei Chen¹  Mingdong Yao¹  Ying Wang¹  Yingjin Yuan¹  Wenhai Xiao² 

[1] Frontier Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering (Ministry of Education), School of Chemical Engineering and Technology, Tianjin University, Tianjin, China;Frontier Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering (Ministry of Education), School of Chemical Engineering and Technology, Tianjin University, Tianjin, China;Georgia Tech Shenzhen Institute, Tianjin University, Shenzhen, China;

关键词: compartmentalization;    terpenoids;    precursor limitation;    cell cytotoxicity;    metabolic engineering;   

DOI  :  10.3389/fbioe.2023.1132244

 received in 2022-12-27, accepted in 2023-02-13,  发布年份 2023

来源: Frontiers

【 摘 要 】

Metabolic engineering strategies for terpenoid production have mainly focused on bottlenecks in the supply of precursor molecules and cytotoxicity to terpenoids. In recent years, the strategies involving compartmentalization in eukaryotic cells has rapidly developed and have provided several advantages in the supply of precursors, cofactors and a suitable physiochemical environment for product storage. In this review, we provide a comprehensive analysis of organelle compartmentalization for terpenoid production, which can guide the rewiring of subcellular metabolism to make full use of precursors, reduce metabolite toxicity, as well as provide suitable storage capacity and environment. Additionally, the strategies that can enhance the efficiency of a relocated pathway by increasing the number and size of organelles, expanding the cell membrane and targeting metabolic pathways in several organelles are also discussed. Finally, the challenges and future perspectives of this approach for the terpenoid biosynthesis are also discussed.

【 授权许可】

Unknown   
Copyright © 2023 Chen, Xiao, Yao, Wang and Yuan.