I329L protein-based indirect ELISA for detecting antibodies specific to African swine fever virus

Frontiers in Cellular and Infection Microbiology

Cellular and Infection Microbiology

Wenchen Qiu¹  Haorui Luan¹  Chunxi Sun¹  Xinya Cao¹  Zhiyong Shen²  Gang Wang³  Jun Peng³ 

[1] College of Veterinary Medicine, Shandong Agricultural University, Tai’an, China;College of Veterinary Medicine, Shandong Agricultural University, Tai’an, China;Breeding Management Department, Beisanxia Husbandry Company Limited, Hegang, China;College of Veterinary Medicine, Shandong Agricultural University, Tai’an, China;Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Shandong Agricultural University, Tai’an, China;

关键词: African swine fever virus;    I329L protein;    prokaryotic expression;    indirect ELISA;    antibody detection;   

DOI  :  10.3389/fcimb.2023.1150042

 received in 2023-01-23, accepted in 2023-05-23,  发布年份 2023

来源: Frontiers

【 摘 要 】

African swine fever (ASF) is a disease that causes severe economic losses to the global porcine industry. As no vaccine or drug has been discovered for the prevention and control of ASF virus (ASFV), accurate diagnosis and timely eradication of infected animals are the primary measures, which necessitate accurate and effective detection methods. In this study, the truncated ASFV I329L (amino acids 70–237), was induced using IPTG and expressed in Escherichia coli cells. The highly antigenic viral protein I329L was used to develop an indirect enzyme-linked immunosorbent assay (iELISA), named I329L-ELISA, which cut-off value was 0.384. I329L-ELISA was used to detect 186 clinical pig serum samples, and the coincidence rate between the indirect ELISA developed here and the commercial kit was 96.77%. No cross-reactivity was observed with CSFV, PRRSV, PCV2, or PRV antibody-positive pig sera, indicating good specificity. Both intra- assay and inter-assay coefficients were below 10%, and the detection sensitivity of the iELISA reached 1:3200. In this study, an iELISA for ASFV antibody detection was developed based on the truncated ASFV I329L protein. Overall, the I329L-ELISA is a user-friendly detection tool that is suitable for ASFV antibody detection and epidemiological surveillance.

【 授权许可】

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Copyright © 2023 Shen, Qiu, Luan, Sun, Cao, Wang and Peng