Piezo1 channels mediate vasorelaxation of uterine arteries from pseudopregnant rats

Frontiers in Physiology

Physiology

Raiana Dos Anjos Moraes¹  Fernanda Priviero²  R. Clinton Webb²  Jaine McKenzie³  Olufunke O. Arishe⁴  Vanessa Dela Justina⁵ 

[1] Department of Cell Biology and Anatomy—School of Medicine, University of South Carolina, Columbia, SC, United States;Cardiovascular Translational Research Center—School of Medicine, University of South Carolina, Columbia, SC, United States;Department of Cell Biology and Anatomy—School of Medicine, University of South Carolina, Columbia, SC, United States;Cardiovascular Translational Research Center—School of Medicine, University of South Carolina, Columbia, SC, United States;College of Engineering and Computing, Biomedical Engineering Program, University of South Carolina, Columbia, MO, United States;Department of General Surgery, Vanderbilt University, Nashville, TN, United States;Department of Physiology, University of Tennessee Health Sciences Center, Memphis, TN, United States;Graduate Program in Biological Sciences, Federal University of Goias, Goiânia, Goias, Brazil;

关键词: Piezo1 channel;    vasorelaxation;    pregnancy;    endothelium;    mechanotransduction;   

DOI  :  10.3389/fphys.2023.1140989

 received in 2023-01-09, accepted in 2023-05-09,  发布年份 2023

来源: Frontiers

【 摘 要 】

Introduction: There is a great increase in uterine arterial blood flow during normal pregnancy, which is a result of the cardiovascular changes that occur in pregnancy to adapt the maternal vascular system to meet the increased metabolic needs of both the mother and the fetus. The cardiovascular changes include an increase in cardiac output and more importantly, dilation of the maternal uterine arteries. However, the exact mechanism for the vasodilation is not fully known. Piezo1 mechanosensitive channels are highly expressed in endothelial and vascular smooth muscle cells of small-diameter arteries and play a role in structural remodeling. In this study, we hypothesize that the mechanosensitive Piezo1 channel plays a role in the dilation of the uterine artery (UA) during pregnancy.Methods: For this, 14-week-old pseudopregnant and virgin Sprague Dawley rats were used. In isolated segments of UA and mesenteric resistance arteries (MRA) mounted in a wire myograph, we investigated the effects of chemical activation of Piezo1, using Yoda 1. The mechanism of Yoda 1 induced relaxation was assessed by incubating the vessels with either vehicle or some inhibitors or in the presence of a potassium-free physiological salt solution (K+-free PSS).Results: Our results show that concentration-dependent relaxation responses to Yoda 1 are greater in the UA of the pseudo-pregnant rats than in those from the virgin rats while no differences between groups were observed in the MRAs. In both vascular beds, either in virgin or in pseudopregnant, relaxation to Yoda 1 was at least in part nitric oxide dependent.Discussion: Piezo1 channel mediates nitric oxide dependent relaxation, and this channel seems to contribute to the greater dilation that occurs in the uterine arteries of pseudo-pregnant rats.

【 授权许可】

Unknown   
Copyright © 2023 Arishe, McKenzie, Dela Justina, Dos Anjos Moraes, Webb and Priviero.