Anatomic and neurochemical analysis of the palpal olfactory system in the red flour beetle Tribolium castaneum, HERBST

Frontiers in Cellular Neuroscience

Cellular Neuroscience

Stefan Dippel¹  Björn Trebels¹  Janet Anders¹  Brigitte Goetz¹  Clara Ernst¹  Tim Keyser¹  Joachim Schachtner²  Karl Heinz Rexer³  Ernst A. Wimmer⁴ 

[1] Animal Physiology, Department of Biology, Philipps-University Marburg, Marburg, Germany;Animal Physiology, Department of Biology, Philipps-University Marburg, Marburg, Germany;Clausthal University of Technology, Clausthal-Zellerfeld, Germany;Biodiversity of Plants, Department of Biology, Philipps-University Marburg, Marburg, Germany;Department of Developmental Biology, Johann-Friedrich-Blumenbach-Institute for Zoology and Anthropology, Georg-August-University Göttingen, Göttingen, Germany;

关键词: Tribolium castaneum;    insect;    olfaction;    chemosensation;    neuroanatomy;    gnathal olfactory center;    lobus glomerulatus;    palpal sensilla;   

DOI  :  10.3389/fncel.2023.1097462

 received in 2022-11-13, accepted in 2023-01-30,  发布年份 2023

来源: Frontiers

【 摘 要 】

The paired antennal lobes were long considered the sole primary processing centers of the olfactory pathway in holometabolous insects receiving input from the olfactory sensory neurons of the antennae and mouthparts. In hemimetabolous insects, however, olfactory cues of the antennae and palps are processed separately. For the holometabolous red flour beetle Tribolium castaneum, we could show that primary processing of the palpal and antennal olfactory input also occurs separately and at distinct neuronal centers. While the antennal olfactory sensory neurons project into the antennal lobes, those of the palps project into the paired glomerular lobes and the unpaired gnathal olfactory center. Here we provide an extended analysis of the palpal olfactory pathway by combining scanning electron micrographs with confocal imaging of immunohistochemical staining and reporter expression identifying chemosensory and odorant receptor-expressing neurons in the palpal sensilla. In addition, we extended the anatomical characterization of the gnathal olfactory center by 3D reconstructions and investigated the distribution of several neuromediators. The similarities in the neuromediator repertoire between antennal lobes, glomerular lobes, and gnathal olfactory center underline the role of the latter two as additional primary olfactory processing centers.

【 授权许可】

Unknown   
Copyright © 2023 Trebels, Dippel, Anders, Ernst, Goetz, Keyser, Rexer, Wimmer and Schachtner.