Changes in the bacterial and microeukaryotic communities in the bioreactor upon increasing heavy metal concentrations

Frontiers in Environmental Chemistry

Environmental Chemistry

Yendi E. Navarro-Noya¹  Nina Montoya-Ciriaco¹  Luc Dendooven²  Mario Hernández-Guzmán²  Gabriel R. Hernández-Martínez²  Frédéric Thalasso²  Claudia E. Aceves-Suriano² 

[1] Centro de Investigación de Ciencias Biológicas, Universidad Autónoma de Tlaxcala, Tlaxcala, Mexico;Laboratory of Soil Ecology, Cinvestav, Mexico City, Mexico;

关键词: 16S rRNA;    18S rRNA;    heavy metals;    bioreactor;    activated sludge;   

DOI  :  10.3389/fenvc.2023.1221820

 received in 2023-05-12, accepted in 2023-08-07,  发布年份 2023

来源: Frontiers

【 摘 要 】

Heavy metals are necessary at low concentration for biological activity, but they are often toxic for microorganisms at high concentrations. Amplicon sequencing of 16S rRNA and 18S rRNA was used to investigate changes in the bacterial and microeukaryotic communities in an activated sludge bioreactor incrementally contaminated with nickel (Ni), copper (Cu), and zinc (Zn) with an IC50 value ranging from 0% to 100%, as previously determined, while an uncontaminated bioreactor served as a control. The chemical oxygen demand (COD) was on average 90% in the uncontaminated bioreactor but dropped to 49% when the heavy metal concentration was 100% IC50. The bacterial community in the uncontaminated bioreactor was dominated by Alphaproteobacteria (mostly Agrobacterium and Brevundimonas) when the heavy metal concentrations were low and Bacteroidetes (mostly Sphingobacterium) when the highest amounts of heavy metal concentrations were applied. The members of Ciliophora, Ascomycota, and Basidiomycota alternatively dominated in the uncontaminated bioreactor, while Ascomycota (mostly Fusarium) dominated in the contaminated bioreactor. The results revealed that increased concentrations of Ni, Cu, and Zn altered the bacterial and microeukaryotic communities and some putative metabolic functions.

【 授权许可】

Unknown   
Copyright © 2023 Aceves-Suriano, Montoya-Ciriaco, Hernández-Guzmán, Hernández-Martínez, Navarro-Noya, Thalasso and Dendooven.