Frontiers in Immunology | |
Inactivated ostreid herpesvirus-1 induces an innate immune response in the Pacific oyster, Crassostrea gigas, hemocytes | |
Immunology | |
Julien Vignier1  Anne Rolton2  Lizenn Delisle2  | |
[1] Aquaculture Group, Cawthron Institute, Nelson, New Zealand;Biosecurity Group, Cawthron Institute, Nelson, New Zealand; | |
关键词: immune priming; Ostreid herpesvirus-1; pseudo-vaccination; innate immune memory; flow cytometry; reactive oxygen species; droplet digital PCR; | |
DOI : 10.3389/fimmu.2023.1161145 | |
received in 2023-02-07, accepted in 2023-04-18, 发布年份 2023 | |
来源: Frontiers | |
【 摘 要 】
Infectious diseases are a major constraint to the expansion of shellfish production worldwide. Pacific oyster mortality syndrome (POMS), a polymicrobial disease triggered by the Ostreid herpesvirus-1 (OsHV-1), has devastated the global Pacific oyster (Crassostrea gigas) aquaculture industry. Recent ground-breaking research revealed that C. gigas possess an immune memory, capable of adaption, which improves the immune response upon a second exposure to a pathogen. This paradigm shift opens the door for developing ‘vaccines’ to improve shellfish survival during disease outbreaks. In the present study, we developed an in-vitro assay using hemocytes – the main effectors of the C. gigas immune system – collected from juvenile oysters susceptible to OsHV-1. The potency of multiple antigen preparations (e.g., chemically and physically inactivated OsHV-1, viral DNA, and protein extracts) to stimulate an immune response in hemocytes was evaluated using flow cytometry and droplet digital PCR to measure immune-related subcellular functions and gene expression, respectively. The immune response to the different antigens was benchmarked against that of hemocytes treated with Poly (I:C). We identified 10 antigen preparations capable of inducing immune stimulation in hemocytes (ROS production and positively expressed immune- related genes) after 1 h of exposure, without causing cytotoxicity. These findings are significant, as they evidence the potential for priming the innate immunity of oysters using viral antigens, which may enable cost-effective therapeutic treatment to mitigate OsHV-1/POMS. Further testing of these antigen preparations using an in-vivo infection model is essential to validate promising candidate pseudo-vaccines.
【 授权许可】
Unknown
Copyright © 2023 Delisle, Rolton and Vignier
【 预 览 】
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RO202310102622490ZK.pdf | 3493KB | download |