Exopolysaccharide is the potential effector of Lactobacillus fermentum PS150, a hypnotic psychobiotic strain

Frontiers in Microbiology

Microbiology

Fu-Sheng Deng¹  Pei-Jun Wen¹  Chien-Chen Wu¹  Hsu-Feng Chu²  Chin-Lin Huang³  Ying-Chieh Tsai⁴  Shao-Ping Chien⁵  Ying-Tsong Chen⁶  Mei-Kuang Lu⁷  Chi-Hsein Chao⁷ 

[1] Bened Biomedical Co., Ltd., Taipei, Taiwan;Biomedical Industry Ph.D. Program, National Yang Ming Chiao Tung University, Taipei, Taiwan;Biomedical Industry Ph.D. Program, National Yang Ming Chiao Tung University, Taipei, Taiwan;Bened Biomedical Co., Ltd., Taipei, Taiwan;Institute of Biochemistry and Molecular Biology, National Yang Ming Chiao Tung University, Taipei, Taiwan;Institute of Genomics and Bioinformatics, National Chung Hsing University, Taichung, Taiwan;Institute of Genomics and Bioinformatics, National Chung Hsing University, Taichung, Taiwan;Institute of Molecular and Genomic Medicine, National Health Research Institutes, Zhunan Town, Miaoli County, Taiwan;National Research Institute of Chinese Medicine, Ministry of Health and Welfare, Taipei, Taiwan;

关键词: exopolysaccharide;    EPS;    heat-killed;    Lactobacillus fermentum;    PS150;    hypnotic;    postbiotic;   

DOI  :  10.3389/fmicb.2023.1209067

 received in 2023-04-20, accepted in 2023-06-05,  发布年份 2023

来源: Frontiers

【 摘 要 】

Psychobiotics are a class of probiotics that confer beneficial effects on the mental health of the host. We have previously reported hypnotic effects of a psychobiotic strain, Lactobacillus fermentum PS150 (PS150), which significantly shortens sleep latency in experimental mice, and effectively ameliorate sleep disturbances caused by either caffeine consumption or a novel environment. In the present study, we discovered a L. fermentum strain, GR1009, isolated from the same source of PS150, and found that GR1009 is phenotypically distinct but genetically similar to PS150. Compared with PS150, GR1009 have no significant hypnotic effects in the pentobarbital-induced sleep test in mice. In addition, we found that heat-killed PS150 exhibited hypnotic effects and altered the gut microbiota in a manner similar to live bacteria, suggesting that a heat-stable effector, such as exopolysaccharide (EPS), could be responsible for these effects. Our comparative genomics analysis also revealed distinct genetic characteristics in EPS biosynthesis between GR1009 and PS150. Furthermore, scanning electron microscopy imaging showed a sheet-like EPS structure in PS150, while GR1009 displayed no apparent EPS structure. Using the phenol-sulfate assay, we found that the sugar content value of the crude extract containing EPS (C-EPS) from PS150 was approximately five times higher than that of GR1009, indicating that GR1009 has a lower EPS production activity than PS150. Through the pentobarbital-induced sleep test, we confirmed the hypnotic effects of the C-EPS isolated from PS150, as evidenced by a significant reduction in sleep latency and recovery time following oral administration in mice. In summary, we utilized a comparative approach to delineate differences between PS150 and GR1009 and proposed that EPS may serve as a key factor that mediates the observed hypnotic effect.

【 授权许可】

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Copyright © 2023 Huang, Chu, Wu, Deng, Wen, Chien, Chao, Chen, Lu and Tsai.