The eNOS isoform exhibits increased expression and activation in the main olfactory bulb of nNOS knock-out mice

Frontiers in Cellular Neuroscience

Neuroscience

David Pérez-Boyero¹  Jorge Valero¹  José Ramón Alonso¹  Eduardo Weruaga¹  Valeria Lorena Cabedo¹  David Díaz¹  Carlos Hernández-Pérez¹ 

[1] Institute for Neuroscience of Castilla and León (INCYL), Universidad de Salamanca, Salamanca, Spain;Institute of Biomedical Research of Salamanca (IBSAL), Salamanca, Spain;

关键词: nitric oxide;    nitric oxide synthase;    olfaction;    olfactory bulb;    main olfactory bulb;    plasticity;   

DOI  :  10.3389/fncel.2023.1120836

 received in 2022-12-10, accepted in 2023-02-27,  发布年份 2023

来源: Frontiers

【 摘 要 】

The main olfactory bulb (MOB) is a neural structure that processes olfactory information. Among the neurotransmitters present in the MOB, nitric oxide (NO) is particularly relevant as it performs a wide variety of functions. In this structure, NO is produced mainly by neuronal nitric oxide synthase (nNOS) but also by inducible nitric oxide synthase (iNOS) and endothelial nitric oxide synthase (eNOS). The MOB is considered a region with great plasticity and the different NOS also show great plasticity. Therefore, it could be considered that this plasticity could compensate for various dysfunctional and pathological alterations. We examined the possible plasticity of iNOS and eNOS in the MOB in the absence of nNOS. For this, wild-type and nNOS knock-out (nNOS-KO) mice were used. We assessed whether the absence of nNOS expression could affect the olfactory capacity of mice, followed by the analysis of the expression and distribution of the NOS isoforms using qPCR and immunofluorescence. NO production in MOB was examined using both the Griess and histochemical NADPH-diaphorase reactions. The results indicate nNOS-KO mice have reduced olfactory capacity. We observed that in the nNOS-KO animal, there is an increase both in the expression of eNOS and NADPH-diaphorase, but no apparent change in the level of NO generated in the MOB. It can be concluded that the level of eNOS in the MOB of nNOS-KO is related to the maintenance of normal levels of NO. Therefore, our findings suggest that nNOS could be essential for the proper functioning of the olfactory system.

【 授权许可】

Unknown   
Copyright © 2023 Pérez-Boyero, Hernández-Pérez, Valero, Cabedo, Alonso, Díaz and Weruaga.