Redistribution of the astrocyte phenotypes in the medial vestibular nuclei after unilateral labyrinthectomy

Frontiers in Neuroscience

Neuroscience

Jie Li¹  Yaqin Wu¹  Jiping Wang¹  Zhengnong Chen¹  Dongzhen Yu¹  Pengjun Wang¹  Haibo Shi²  Shankai Yin²  Lu-Yang Wang³ 

[1] Department of Otorhinolaryngology—Head and Neck Surgery, Shanghai Sixth People’s Hospital affiliated to Shanghai Jiaotong University School of Medicine, Shanghai, China;Department of Otorhinolaryngology—Head and Neck Surgery, Shanghai Sixth People’s Hospital affiliated to Shanghai Jiaotong University School of Medicine, Shanghai, China;Shanghai Key Laboratory of Sleep Disordered Breathing, Shanghai, China;Programs in Neurosciences & Mental Health, SickKids Research Institute and Department of Physiology, University of Toronto, Toronto, ON, Canada;

关键词: vestibular compensation;    labyrinthectomy;    astrocyte;    single-cell RNA seq;    plasticity;   

DOI  :  10.3389/fnins.2023.1146147

 received in 2023-01-17, accepted in 2023-06-05,  发布年份 2023

来源: Frontiers

【 摘 要 】

Astrocytes are highly heterogeneous and involved in different aspects of fundamental functions in the central nervous system (CNS). However, whether and how this heterogeneous population of cells reacts to the pathophysiological challenge is not well understood. To investigate the response status of astrocytes in the medial vestibular nucleus (MVN) after vestibular loss, we examined the subtypes of astrocytes in MVN using single-cell sequencing technology in a unilateral labyrinthectomy mouse model. We discovered four subtypes of astrocytes in the MVN with each displaying unique gene expression profiles. After unilateral labyrinthectomy, the proportion of the astrocytic subtypes and their transcriptional features on the ipsilateral side of the MVN differ significantly from those on the contralateral side. With new markers to detect and classify the subtypes of astrocytes in the MVN, our findings implicate potential roles of the adaptive changes of astrocyte subtypes in the early vestibular compensation following peripheral vestibular damage to reverse behavioral deficits.

【 授权许可】

Unknown   
Copyright © 2023 Li, Wang, Wang, Wu, Wang, Yu, Chen, Shi and Yin.