Entomopathogenic fungus Beauveria bassiana–based bioinsecticide suppresses severity of powdery mildews of vegetables by inducing the plant defense responses

Frontiers in Plant Science

Plant Science

Satoshi Yamanaka¹  Katsumi Kamiya²  Kazuya Maeda³  Mariko Kouda³  Yuichiro Iida⁴  Ayano Sasaki⁵  Chiharu Tanaka⁵  Taku Kawakami⁵  Kaori Nakajima⁵  Katsutoshi Kuroda⁵  Naho Yamagishi⁶  Masashi Fujinaga⁶  Kandai Yoshida⁷  Shunsuke Asano⁷  Fumihiro Terami⁸  Oumi Nishi⁸  Yumiko Higashi⁸  Masaharu Kubota⁹ 

[1] Arysta LifeScience Corporation, Tokyo, Japan;Gifu Prefectural Agricultural Technology Center, Gifu, Japan;Laboratory of Plant Pathology, Faculty of Agriculture, Setsunan University, Hirakata, Japan;Laboratory of Plant Pathology, Faculty of Agriculture, Setsunan University, Hirakata, Japan;National Agriculture and Food Research Organization, Tsu, Japan;Mie Prefecture Agricultural Research Institute, Matsusaka, Japan;Nagano Vegetable and Ornamental Crops Experiment Station, Shiojiri, Japan;Nara Prefecture Agricultural Research and Development Center, Sakurai, Japan;National Agriculture and Food Research Organization, Tsu, Japan;National Agriculture and Food Research Organization, Tsukuba, Japan;

关键词: endophyte;    biofungicide;    dual control;    biocontrol agent;    salicylic acid;    induced resistance;    plant-microbe interaction;    Podosphaera xanthii;   

DOI  :  10.3389/fpls.2023.1211825

 received in 2023-04-25, accepted in 2023-08-07,  发布年份 2023

来源: Frontiers

【 摘 要 】

The entomopathogenic fungus Beauveria bassiana is used commercially as a microbial insecticides against a wide range of agricultural insect pests. Some strains of B. bassiana protect the plants from pathogens, but the underlying mechanisms are largely unknown. Here, we found that prophylactic sprays of commercial bioinsecticide Botanigard on cucumber, tomato, and strawberry plants suppressed the severity of economically damaging powdery mildews. On leaf surfaces, hyphal elongation and spore germination of cucumber powdery mildew, Podosphaera xanthii, were inhibited, but B. bassiana strain GHA, the active ingredient isolated from Botanigard, only inhibited hyphal elongation but had no effect on spore germination of P. xanthii. In addition, strain GHA suppressed powdery mildew symptoms locally, not systemically. Treatment with Botanigard and strain GHA induced a hypersensitive response (HR)–like cell death in epidermal cells of the cucumber leaves in a concentration-dependent manner and inhibited penetration by P. xanthii. Transcriptome analysis and mass spectrometry revealed that GHA induced expression of salicylic acid (SA)–related genes, and treatment with Botanigard and GHA increased the SA level in the cucumber leaves. In NahG-transgenic tomato plants, which do not accumulate SA, the biocontrol effect of tomato powdery mildew by GHA was significantly reduced. These results suggested that B. bassiana GHA induces SA accumulation, leading to the induction of HR-like cell death against powdery mildew and subsequent suppression of fungal penetration. Thus, Botanigard has the potential to control both insect pests and plant diseases.

【 授权许可】

Unknown   
Copyright © 2023 Iida, Higashi, Nishi, Kouda, Maeda, Yoshida, Asano, Kawakami, Nakajima, Kuroda, Tanaka, Sasaki, Kamiya, Yamagishi, Fujinaga, Terami, Yamanaka and Kubota