Reaching unreachables: Obstacles and successes of microbial cultivation and their reasons

Frontiers in Microbiology

Microbiology

Marco A. Lopez Marin¹  Gabriela Kapinusova¹  Ondrej Uhlik² 

[1] Department of Biochemistry and Microbiology, Faculty of Food and Biochemical Technology, University of Chemistry and Technology, Prague, Czechia;null;

关键词: environmental microbiome;    microbial ecology;    dormancy;    VBNC;    growth factors;    cultivation techniques;    improved cultivation;    difficult-to-culture microorganisms;   

DOI  :  10.3389/fmicb.2023.1089630

 received in 2022-11-04, accepted in 2023-02-10,  发布年份 2023

来源: Frontiers

【 摘 要 】

In terms of the number and diversity of living units, the prokaryotic empire is the most represented form of life on Earth, and yet it is still to a significant degree shrouded in darkness. This microbial “dark matter” hides a great deal of potential in terms of phylogenetically or metabolically diverse microorganisms, and thus it is important to acquire them in pure culture. However, do we know what microorganisms really need for their growth, and what the obstacles are to the cultivation of previously unidentified taxa? Here we review common and sometimes unexpected requirements of environmental microorganisms, especially soil-harbored bacteria, needed for their replication and cultivation. These requirements include resuscitation stimuli, physical and chemical factors aiding cultivation, growth factors, and co-cultivation in a laboratory and natural microbial neighborhood.

【 授权许可】

Unknown   
Copyright © 2023 Kapinusova, Lopez Marin and Uhlik.