3D printed hybrid scaffolds for bone regeneration using calcium methoxyethoxide as a calcium source

Frontiers in Bioengineering and Biotechnology

Bioengineering and Biotechnology

Julian R. Jones¹  Agathe Heyraud¹  Hung-Kai Ting¹  Francesca Tallia¹  Anna Tchorzewska¹  Jingwen Liu²  Peter D. Lee²  Hannah L. Pilsworth³  John V. Hanna³  Sara M. Rankin⁴  David Sory⁴ 

[1] Department of Materials, Imperial College London, London, United Kingdom;Department of Mechanical Engineering, Faculty of Engineering Science, University College London, London, United Kingdom;Department of Physics, University of Warwick, Coventry, United Kingdom;Faculty of Medicine, Imperial College London, National Heart and Lung Institute, London, United Kingdom;

关键词: sol-gel hybrid;    bone regeneration;    bioactive;    Direct Ink Writing;    calcium;   

DOI  :  10.3389/fbioe.2023.1224596

 received in 2023-05-17, accepted in 2023-08-03,  发布年份 2023

来源: Frontiers

【 摘 要 】

Introduction: Hybrids consist of inorganic and organic co-networks that are indistinguishable above the nanoscale, which can lead to unprecedented combinations of properties, such as high toughness and controlled degradation.Methods: We present 3D printed bioactive hybrid scaffolds for bone regeneration, produced by incorporating calcium into our “Bouncy Bioglass”, using calcium methoxyethoxide (CME) as the calcium precursor. SiO2-CaOCME/PTHF/PCL-diCOOH hybrid “inks” for additive manufacturing (Direct Ink Writing) were optimised for synergy of mechanical properties and open interconnected pore channels.Results and Discussion: Adding calcium improved printability. Changing calcium content (5, 10, 20, 30, and 40 mol.%) of the SiO2-CaOCME/PTHF/PCL-diCOOH hybrids affected printability and mechanical properties of the lattice-like scaffolds. Hybrids containing 30 mol.% calcium in the inorganic network (70S30CCME-CL) printed with 500 µm channels and 100 µm strut size achieved the highest strength (0.90 ± 0.23 MPa) and modulus of toughness (0.22 ± 0.04 MPa). These values were higher than Ca-free SiO2/PTHF/PCL-diCOOH hybrids (0.36 ± 0.14 MPa strength and 0.06 ± 0.01 MPa toughness modulus). Over a period of 90 days of immersion in simulated body fluid (SBF), the 70S30CCME-CL hybrids also kept a stable strain to failure (~30 %) and formed hydroxycarbonate apatite within three days. The extracts released by the 70S30CCME-CL hybrids in growth medium did not cause cytotoxic effects on human bone marrow stromal cells over 24 h of culture.

【 授权许可】

Unknown   
Copyright © 2023 Heyraud, Tallia, Sory, Ting, Tchorzewska, Liu, Pilsworth, Lee, Hanna, Rankin and Jones.