Haplotype Analysis of Chloroplast Genomes for Jujube Breeding

Frontiers in Plant Science

Plant Science

Yang Wu¹  Ningguang Dong¹  Chaojun Guo¹  Bo Chen¹  Dongye Lu¹  Guanglong Hu¹  Yanjie Qiao¹  Yuping Zhang²  Qinghua Pan² 

[1] Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (North China), Ministry of Agriculture, Beijing Engineering Research Center for Deciduous Fruit Trees, Institute of Forestry and Pomology, Beijing Academy of Agriculture and Forestry Sciences, Beijing, China;null;

关键词: Ziziphus jujuba;    chloroplast genome;    genomic structure;    phylogenetic analysis;    evolutionary relationship;    breeding strategy;   

DOI  :  10.3389/fpls.2022.841767

 received in 2021-12-22, accepted in 2022-02-11,  发布年份 2022

来源: Frontiers

【 摘 要 】

Jujube (family Rhamnaceae) is an important economic fruit tree in China. In this study, we reported 26 chloroplast (cp) sequences of jujube using Illumina paired-end sequencing. The sequence length of cp genome was 161, 367–161, 849 bp, which was composed of a large single-copy region (89053–89437 bp) and a small single-copy region (19356–19362 bp) separated by a pair of reverse repeat regions (26478–26533 bp). Each cp genome encodes the same 130 genes, including 112 unique genes, being quite conserved in genome structure and gene sequence. A total of 118 single base substitutions (SNPs) and 130 InDels were detected in 65 jujube accessions. Phylogenetic and haplotype network construction methods were used to analyze the origin and evolution of jujube and its sour-tasting relatives. We detected 32 effective haplotypes, consisting of 20 unique jujube haplotypes and 9 unique sour–jujube haplotypes. Compared with sour–jujube, jujube showed greater haplotype diversity at the chloroplast DNA level. To cultivate crisp and sweet fruit varieties featuring strong resistance, by combining the characteristics of sour-jujube and cultivated jujube, three hybrid combinations were suggested for reciprocal crosses: “Dongzao” × “Jingzao39,” “Dongzao” × “Jingzao60,” “Dongzao” × “Jingzao28.” This study provides the basis for jujube species’ identification and breeding, and lays the foundation for future research.

【 授权许可】

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Copyright © 2022 Hu, Wu, Guo, Lu, Dong, Chen, Qiao, Zhang and Pan.