Analysis of changes in bacterial diversity in healthy and bacterial wilt mulberry samples using metagenomic sequencing and culture-dependent approaches

Frontiers in Plant Science

Plant Science

Weili Liu¹  Peijia Yang¹  Hongyu Yang¹  Ting Yuan¹  Izhar Hyder Qazi¹  Jinhao Li¹  Jiping Liu¹  Xueyin Zhang¹ 

[1] South China Agriculture University, College of Animal Science, Regional Sericulture Training Center for Asia-Pacific, Guangzhou, Guangdong, China;

关键词: bacterial wilt;    drug-resistant bacteria;    Enterobacter cloacae;    Klebsiella;    mulberry;    opportunistic pathogens;    RSSC;   

DOI  :  10.3389/fpls.2023.1206691

 received in 2023-04-16, accepted in 2023-07-21,  发布年份 2023

来源: Frontiers

【 摘 要 】

IntroductionMulberry bacterial wilt is a serious destructive soil-borne disease caused by a complex and diverse group of pathogenic bacteria. Given that the bacterial wilt has been reported to cause a serious damage to the yield and quality of mulberry, therefore, elucidation of its main pathogenic groups is essential in improving our understanding of this disease and for the development of its potential control measures.MethodsIn this study, combined metagenomic sequencing and culture-dependent approaches were used to investigate the microbiome of healthy and bacterial wilt mulberry samples.ResultsThe results showed that the healthy samples had higher bacterial diversity compared to the diseased samples. Meanwhile, the proportion of opportunistic pathogenic and drug-resistant bacterial flora represented by Acinetobacter in the diseased samples was increased, while the proportion of beneficial bacterial flora represented by Proteobacteria was decreased. Ralstonia solanacearum species complex (RSSC), Enterobacter cloacae complex (ECC), Klebsiella pneumoniae, K. quasipneumoniae, K. michiganensis, K. oxytoca, and P. ananatis emerged as the main pathogens of the mulberry bacterial wilt.DiscussionIn conclusion, this study provides a valuable reference for further focused research on the bacterial wilt of mulberry and other plants.

【 授权许可】

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Copyright © 2023 Yuan, Qazi, Li, Yang, Yang, Zhang, Liu and Liu